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Volume 19, Number 2—February 2013

Letter

Clustered Cases of Rickettsia sibirica mongolitimonae Infection, France

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To the Editor: Rickettsia sibirica mongolitimonae, a member of the tick-borne spotted fever group (SFG) of rickettsia, was first isolated from Hyalomma asiaticum ticks in China (1). The first human case was described in France in 1996, and 7 new cases were described in 2005 (1). This rickettsiosis was named lymphangitis-associated rickettsiosis because lymphangitis was observed in 50% of the patients (1). Only 17 cases have been reported, for which 7 patients had lymphangitis, and 13 had inoculation eschars, including 2 patients with 2 eschars (1,2). We report a cluster of cases of R. sibirica mongolitimonae infection.

Patient 1, a 73-year-old man in France, had fever, rash, lymphadenopathies, and an axillary inoculation eschar in February 2011. A diagnosis of lymphangitis-associated rickettsiosis was suspected because of the season (most cases occur in spring in France) and clinical manifestations. The patient was confined to bed for several weeks after surgical placement of a knee prosthesis when his disease occurred; the domestic cat was suspected to have introduced ticks into the home.

In April, his wife (67 years of age) (patient 2) became febrile, had popliteal lymphadenopathies associated with lymphangitis, and had an eschar on the leg from which a swab specimen was obtained. Patient 3 was their neighbor; he had the same symptoms in March 2011 but samples were not collected from him.

None of patients reported tick bites, but they were in regular contact with animals, including a cat, a dog, horses, and birds. Both patients who lived with the cat reported that it would return home with ticks. Infections in these patients were successfully treated with doxycycline.

An immunofluorescence assay for antibodies against SFG antigens showed IgG/IgM titers of 128/0 for patient 1 and 64/16 for patient 2 (3). DNA was extracted from the skin swab specimen of patient 2 by using the QIAamp Mini Kit (QIAGEN, Hilden, Germany). A fragment of the citrate synthase gene of Rickettsia spp. was amplified by PCR and sequenced. The sequence show 99.7% homology with that of the same gene sequence of R. sibirica mongolitimonae in GenBank (accession no. DQ423370) (4).

Ticks were collected from the property of the 2 patients: from the garden by flagging vegetation (3), from animals, and near the cat litter (Table). SFG rickettsiae were detected by specific quantitative PCR. Species identification was confirmed by specific quantitative PCR for R. massiliae and sequencing of outer membrane protein A gene for others species (5). A negative control (sterile water) and positive control (DNA from R. montanensis or R. massiliae) were included in each PCR.

Ticks were morphologically identified as adult Rhipicephalus sanguineus. Molecular identification of these ticks harboring rickettsiae was performed by amplification of the 12S rRNA gene. DNA from R. massiliae was found in 3 ticks collected from the dog and near the cat litter morphologically identified as Rh. sanguineus. This DNA showed 98% homology with the sequence in GenBank (accession no. AY559843). R. sibirica mongolitimonae with 99.8% homology for the outer membrane protein gene sequence in GenBank (accession no. DQ097082) was isolated from 1 tick collected from the cat. This tick was identified as Rh. pusillus and showed 99.7% homology with the sequence in GenBank (accession no. FJ536547). R. massiliae was cultured from an Rh. sanguineus tick, and R. sibirica mongolitimonae was cultured from an Rh. pusillus tick.

A cluster of 1 documented case and 2 probable cases of lymphangitis-associated rickettsiosis in southern France was linked to a cat and Rh. pusillus ticks. Infection with R. massiliae for the 2 probable case-patients was unlikely because clinical findings were typical of lymphangitis-associated rickettsiosis, and most cases of rickettsioses in southern France in the spring are caused by R. sibirica mongolitimonae. Clustered cases of SFG rickettsiae infection have been reported in Europe, including southern France (3,6). In 2007, R. conorii and R. massiliae infections in humans were reported (3). In 2010, cases for which we were unable to discriminate between R. conorii and R. massiliae infections occurred in a family (6). In these 2 studies, clustered cases of SFG rickettsiosis involved Rh. sanguineus ticks. Clustered cases appeared to be related to an increase in aggressiveness of ticks toward humans during warmer periods (3). In our study, no correlation was identified with warmer weather.

R. sibirica mongolitimonae is most frequently associated with Hyalomma spp. ticks (1,2,4). However, 1 case of infection with this bacterium was associated with Rh. pusillus ticks collected in Portugal (7); DNA from this bacteria was also identified in an Rh. pusillus tick collected from a mongoose. The European wild rabbit is the primary host of Rh. pusillus ticks. However, these ticks have been found on wild carnivorous animals, dogs, and domestic cats (8); these ticks can bite humans (8). Moreover, R. massiliae and R. sibirica mongolitimonae were found in Rh. pusillus ticks from Spain (9), and SFG rickettsiae were found in ticks from Sardinia (10). Therefore, Rh. pusillus ticks appear to be an emerging vector for R. sibirica mongolitimonae in Europe.

Sophie Edouard, Philippe Parola, Cristina Socolovschi, Bernard Davoust, Bernard La Scola, and Didier RaoultComments to Author 
Author affiliations: Author affiliation: Aix-Marseille Université, Marseille, France

References

  1. Fournier PE, Gouriet F, Brouqui P, Lucht F, Raoult D. Lymphangitis-associated rickettsiosis, a new rickettsiosis caused by Rickettsia sibirica mongolotimonae: seven new cases and review of the literature. Clin Infect Dis. 2005;40:143544 and. DOIPubMed
  2. Socolovschi C, Barbarot S, Lefebvre M, Parola P, Raoult D. Rickettsia sibirica mongolitimonae in traveler from Egypt. Emerg Infect Dis. 2010;16:14956 and. DOIPubMed
  3. Parola P, Socolovschi C, Jeanjean L, Bitam I, Fournier PE, Sotto A, Warmer weather linked to tick attack and emergence of severe rickettsioses. PLoS Negl Trop Dis. 2008;2:e338 and. DOIPubMed
  4. Mediannikov O, Diatta G, Fenollar F, Sokhna C, Trape JF, Raoult D. Tick-borne rickettsioses, neglected emerging diseases in rural Senegal. PLoS Negl Trop Dis. 2010;4:e821 and. DOIPubMed
  5. Renvoisé A, Rolain JM, Socolovschi C, Raoult D. Widespread use of real-time PCR for rickettsial diagnosis. FEMS Immunol Med Microbiol. 2012;64:1269 and. DOIPubMed
  6. Renvoisé A, Delaunay P, Blanchouin E, Cannavo I, Cua C, Socolovschi C, Urban family cluster of spotted fever rickettsiosis linked to Rhipicephalus sanguineus infected with Rickettsia conorii subsp. caspia and Rickettsia massiliae. Ticks Tick Borne Dis 2012; 3:389–92. DOI
  7. de Sousa R, Barata C, Vitorino L, Santos-Silva M, Carrapato C, Torgal J, Rickettsia sibirica isolation from a patient and detection in ticks, Portugal. Emerg Infect Dis. 2006;12:11038 and. DOIPubMed
  8. Santos-Silva MM, Beati L, Santos AS, de Sousa R, Nuncio MS, Melo P, The hard-tick fauna of mainland Portugal (Acari: Ixodidae): an update on geographical distribution and known associations with hosts and pathogens. Exp Appl Acarol. 2011;55:85121 and. DOIPubMed
  9. Toledo A, Olmeda AS, Escudero R, Jado I, Valcarcel F, Casado-Nistal MA, Tick-borne zoonotic bacteria in ticks collected from central Spain. Am J Trop Med Hyg. 2009;81:6774 .PubMed
  10. Satta G, Chisu V, Cabras P, Fois F, Masala G. Pathogens and symbionts in ticks: a survey on tick species distribution and presence of tick-transmitted micro-organisms in Sardinia, Italy. J Med Microbiol. 2011;60:638 and. DOIPubMed

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Suggested citation for this article: Edouard S, Parola P, Socolovschi C, Davoust B, La Scola B, Raoult D. Clustered cases of Rickettsia sibirica mongolitimonae infection, France [letter]. Emerg Infect Dis [Internet]. 2013 Feb [date cited]. http://dx.doi.org/10.3201/eid1902.120863

DOI: 10.3201/eid1902.120863

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Table of Contents – Volume 19, Number 2—February 2013

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Didier Raoult, Unité des Rickettsies, Unité de Recherche sur les Maladies Infectieuses et Tropicales Émergentes, Centre National de la Recherche Scientifique–Institut de Recherche pour le Développement, Unités Mixtes de Recherché 6236, Faculté de Médecine et de Pharmacie, 27 Blvd Jean Moulin 13385 Marseille Cedex 05, France

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