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Volume 19, Number 3—March 2013

Dispatch

Anisakiasis and Gastroallergic Reactions Associated with Anisakis pegreffii Infection, Italy

Simonetta MattiucciComments to Author , Paolo Fazii, Alba De Rosa, Michela Paoletti, Angelo Salomone Megna, Antonio Glielmo, Maurizio De Angelis, Antonella Costa, Costantino Meucci, Vito Calvaruso, Italo Sorrentini, Giuseppe Palma, Fabrizio Bruschi, and Giuseppe Nascetti
Author affiliations: Author affiliations: Sapienza University of Rome, Rome, Italy (S. Mattiucci, A. De Rosa); Umberto I Hospital, Rome (S. Mattiucci, M. De Angelis); Hospital S. Spirito, Pescara, Italy (P. Fazii); Tuscia University, Viterbo, Italy (M. Paoletti, G. Nascetti); G. Rummo Hospital, Benevento, Italy (A. Salomone Megna, A. Glielmo, I. Sorrentini); Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Italy (A. Costa); A. Maresca Hospital, Naples, Italy (C. Meucci); F. Lotti Hospital, Pontedera, Pisa, Italy (V. Calvaruso); The National Federation of Fishery Companies, Rome (G. Palma); University of Pisa, Pisa (F. Bruschi)

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Figure 2

Western blot reaction of the sera from patients HuC1 and HuC2 from Italy showing allergic reaction against Anisakis pegreffii antigens and allergens. M indicates molecular marker; arrow indicates the reaction at 24 kDa (Ani s1). IgE determination was performed with alkaline phosphatase conjugates obtained from goat anti-human IgE. Antigen-antibody binding was visualized by the alkaline phosphatase 5-bromo-4-chloro-3-indolyl phosphate p-nitroblue tetrazolium chloride system until bands appeared.

Figure 2. . . Western blot reaction of serum samples from patients HuC1 and HuC2 from Italy showing allergic reaction against Anisakis pegreffii antigens and allergens. M indicates molecular marker; arrow indicates the reaction at 24 kDa (Ani s1). IgE determination was performed with alkaline phosphatase conjugates obtained from goat anti-human IgE. Antigen-antibody binding was visualized by the alkaline phosphatase 5-bromo-4-chloro-3-indolyl phosphate p-nitroblue tetrazolium chloride system until bands appeared. Human serum specimens negative for A. pegreffii were used as controls. HC1 and HC2 represent patient identification numbers HuC1 and HuC2.

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