Occult Hepatitis B Virus Infection in Chacma Baboons, South Africa
Caroline Dickens, Michael C. Kew, Robert H. Purcell, and Anna Kramvis
Author affiliations: University of the Witwatersrand, Johannesburg, South Africa (C. Dickens, M.C. Kew, A. Kramvis); Groote Schuur Hospital, Cape Town, South Africa (M.C. Kew); National Institutes of Health, Bethesda, Maryland, USA (R.H. Purcell)
Figure 1. . . Amplification of the hepatitis B virus HBV genome by using overlapping subgenomic fragments. Shown are 8 overlapping subgenomic fragments amplified by nested PCR, These fragments were used to generate the complete HBV sequence isolated from liver tissue of Chacma baboon 9732, South Africa. Dashed gray boxes indicate first-round PCRs, and white boxes indicate second-round PCRs. Values along the 2-headed arrow at the top are in basepairs from the EcoRI site of the circular genome of HBV. Small arrows within boxes indicate the direction of amplification.
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