Full-Genome Deep Sequencing and Phylogenetic Analysis of Novel Human Betacoronavirus
Figure 1. . . . A) Primers designed for reverse transcription and overlapping PCR amplification of the novel coronavirus (CoV). Dots indicate the predicted binding site of each primer along the EMC/2012 genome (x-axis). Gray bars indicate predicted amplicon lengths. Amplicon numbers are indicated beside each set of products. B) PCR products (3 µL of a 25-µL reaction) were resolved by electrophoresis on a 0.6% agarose gel and visualized by ethidium bromide staining. Lane M is the molecular weight marker (sizes indicated at left), Lanes 1–15 show the products of the amplicons depicted in Panel A. Lane C is the reagent PCR control.