TY - JOUR AU - Cotten, Matthew AU - Lam, Tommy AU - Watson, Simon AU - Palser, Anne AU - Petrova, Velislava AU - Grant, Paul AU - Pybus, Oliver AU - Rambaut, Andrew AU - Guan, Yi AU - Pillay, Deenan AU - Kellam, Paul AU - Nastouli, Eleni T1 - Full-Genome Deep Sequencing and Phylogenetic Analysis of Novel Human Betacoronavirus T2 - Emerging Infectious Disease journal PY - 2013 VL - 19 IS - 5 SP - 736 SN - 1080-6059 AB - A novel betacoronavirus associated with lethal respiratory and renal complications was recently identified in patients from several countries in the Middle East. We report the deep genome sequencing of the virus directly from a patient’s sputum sample. Our high-throughput sequencing yielded a substantial depth of genome sequence assembly and showed the minority viral variants in the specimen. Detailed phylogenetic analysis of the virus genome (England/Qatar/2012) revealed its close relationship to European bat coronaviruses circulating among the bat species of the Vespertilionidae family. Molecular clock analysis showed that the 2 human infections of this betacoronavirus in June 2012 (EMC/2012) and September 2012 (England/Qatar/2012) share a common virus ancestor most likely considerably before early 2012, suggesting the human diversity is the result of multiple zoonotic events. KW - disease transmission KW - infectious KW - coronavirus infections KW - evolution KW - molecular KW - respiratory tract infections KW - high-throughput nucleotide sequencing KW - computer-aided design KW - viruses KW - zoonoses KW - Qatar KW - Saudi Arabia KW - United Kingdom DO - 10.3201/eid1905.130057 UR - https://wwwnc.cdc.gov/eid/article/19/5/13-0057_article ER - End of Reference