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Volume 19, Number 7—July 2013

Research

Mutation in Spike Protein Cleavage Site and Pathogenesis of Feline Coronavirus

Beth N. Licitra1, Jean K. Millet1, Andrew D. Regan, Brian S. Hamilton, Vera D. Rinaldi, Gerald E. Duhamel, and Gary R. WhittakerComments to Author 
Author affiliations: Cornell University College of Veterinary Medicine, Ithaca, New York, USA

Main Article

Figure 2

Sequence analysis of feline infectious peritonitis virus (FIPV) spike S1/S2 site. RNA from 22 FIPVs collected from 11 cats who had feline infectious peritonitis was extracted, purified, and reverse-transcribed into cDNA. Sequencing of the spike gene was performed in a region surrounding the S1/S2 cleavage site. A) Sequence alignment. Sequence identification row (blue font): residue positions in the S1/S2 cleavage site from P8 to P4′. Red arrow indicates the site of furin cleavage. B) To visualiz

Figure 2. . Sequence analysis of feline infectious peritonitis virus (FIPV) spike S1/S2 site. RNA from 22 FIPVs collected from 11 cats who had feline infectious peritonitis was extracted, purified, and reverse-transcribed into cDNA. Sequencing of the spike gene was performed in a region surrounding the S1/S2 cleavage site. A) Sequence alignment. Sequence identification row (blue font): residue positions in the S1/S2 cleavage site from P8 to P4′. Red arrow indicates the site of furin cleavage. B) To visualize the diversity of residues at each position of the S1/S2 site, sequences were subjected to WebLogo 3.1 analysis (http://weblogo.threeplusone.com/create.cgi). Top: WebLogo for the 22 FIPV S1/S2 sequences with the frequency of residue found at each position displayed. Bottom: summary of the diversity of residues for each position from P4 to P1′ and percentages of each amino acid represented.

Main Article

1These authors contributed equally to this article.

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