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Volume 19, Number 7—July 2013

Letter

Verona Integron–encoded Metallo-β-Lactamase 1 in Enterobacteria, Ontario, Canada

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To the Editor: Among Enterobacteriaceae, Verona integron–encoded metallo-β-lactamase 1 (VIM-1) has been found only in Klebsiella pneumoniae in North America (1). We report 4 VIM-1–producing Enterobacteriaceae isolated from 4 patients at 3 hospitals in Ontario, Canada.

Patient 1, a 61-year-old man, was initially hospitalized in Italy for presumed pneumonia and was treated with levofloxacin during his 6-month stay in Italy. Upon returning to Ontario, Canada, he was admitted to hospital 1 in August 2010 because of diabetic ketoacidosis and began empiric treatment with metronidazole and gentamicin. Urine cultures were positive for a carbapenem-resistant Escherichia coli (strain GN531). Two days later, the patient had a fever and a blood culture was positive for E. coli (strain GN532), which was also resistant to carbapenems. During his hospitalization, the patient was isolated and received droplet precaution because of his travel history until he was discharged home.

Patient 2, a 76-year-old man, was admitted to hospital 2 in May 2011 because of a recurrent urinary tract infection (urine was positive for E. coli). The patient was given ciprofloxacin. On day 49, a carbapenem-sensitive Enterobacter cloacae was isolated from urine. On day 61, a carbapenem-resistant E. cloacae was isolated from urine culture (strain GN719). Contact precautions were used until the patient was discharged to a long-term care facility on day 80.

Patient 3, an 81-year-old man, was admitted to hospital 2 (November 2011) 2 months after patient 2 was discharged. Urine culture at admission was positive for a carbapenem-resistant E. cloacae (strain GN825). The patient was given ceftriaxone and metronidazole and then given ertapenem. The patient died on day 110. Patients 2 and 3 had no hospital room in common during their admissions and both received contact precautions for methicillin-resistant Staphylococcus aureus before isolation of the carbapenem-resistant isolates.

Patient 4, a 90-year-old woman, was admitted to hospital 3 in November 2011 because of nausea, vomiting, and diarrhea. In the preceding 6-month period, she had recurrent Clostridium difficile–associated diarrhea and a urinary tract infection. At admission, a carbapenem-susceptible Proteus spp. was isolated from a urine culture. The patient was given a 3-day course of ciprofloxacin and vancomycin. On day 17, a carbapenem-resistant E. cloacae was isolated from urine (strain GN738). Because this organism was also isolated from a rectal swab specimen, it was assumed that the urine sample might be contaminated by her feces. Therefore, the patient did not receive additional treatment other than that for recurrent C. difficile–associated diarrhea.

Patients 2, 3, and 4 had no history of travel outside Canada. All 5 isolates were submitted for reference purposes to the Public Health Ontario Laboratories. Pulsed-field gel electrophoresis showed that E. coli GN531 and GN532 were indistinguishable (GN531 was selected for further studies), and the 3 E. cloacae isolates had similar fingerprint patterns. All strains displayed synergy in presence of meropenem disks plus dipicolinic acid, which is indicative of metallo-β-lactamase inhibition (2). The 4 clinical strains displayed a multidrug resistance phenotype, and were susceptible only to tigecycline and colistin (Table).

PCR and sequencing identified blaVIM-1 in all isolates (Table). Multilocus sequence typing classified E. coli GN531 as sequence type (ST) 131 (6), the epidemic strain that spreads blaCTX-M-15 worldwide (7). E. coli ST131 with similar phenotypic and genetic features was described in Florence, Italy, in 2009 (8). Because E. coli GN531 was isolated from patient 1, who had received heath care in Italy before being hospitalized in Ontario, this patient might have been exposed to this strain in Italy. A similar scenario was reported in the first case of VIM-1–producing K. pneumoniae in the United States, which was isolated from a patient who received health care in Greece (1). The presence of a metallo-β-lactamase in E. coli ST131 is of great concern because it increases the potential for dissemination of drug-resistance genes.

An IncN plasmid (5) harboring blaVIM-1 was transferred from GN531 to E. coli by conjugation (Table). The blaCTX-M-15 gene was not co-transferred, which indicated that it was located on another plasmid or the chromosome of the clinical isolate. After several attempts, no transconjugants derived from E. cloacae were obtained. E. coli TOP10 (Life Technologies, Carlsbad, CA, USA) was transformed with VIM-1 plasmids obtained from E. cloacae GN719 and GN825 (T-719 and T-825, respectively). E. coli transformation with plasmid extracts from E. cloacae GN738 was unsuccessful. Pulsed-field gel electrophoresis with S1 nuclease (9) and Southern blot analysis identified VIM-1-containing plasmids; estimated sizes were 65 kb (E. coli GN531), 50 kb (E. cloacae GN738), and 30 kb (E. cloacae GN719 and GN825).

In conclusion, VIM-1 was found among Enterobacteriaceae from 3 geographically distant nosocomial units in Ontario, Canada. Although E. cloacae strains were clonally related, there were no clear epidemiologic links between these patients, suggesting that the clone or resistance gene maybe circulating in the province on a greater scale than believed. Emergence of E. coli ST131, a pandemic multidrug-resistant clone that causes predominantly community-onset infections (7), and produces simultaneously CTX-M-15 and VIM-1, could be a serious threat for the dissemination of these drug-resistance elements.

Nathalie Tijet, Gregory Macmullin, Olga Lastovetska, Christie Vermeiren, Patricia Wenzel, Tina Stacey-Works, Donald E. Low, Samir N. Patel, and Roberto G. MelanoComments to Author 
Author affiliations: Public Health Ontario Laboratories–Toronto, Toronto, Ontario, Canada (N. Tijet, G. Macmullin, O. Latovetska, D.E. Low, S.N. Patel, R.G. Melano); University of Toronto, Toronto (O. Lastovetska, D.E. Low, S.N. Patel, R.G. Melano); Shared Hospital Laboratory, Toronto (C. Vermeiren); Halton Healthcare Services, Oakville, Ontario, Canada (P. Wenzel, T. Stacey-Works); Mount Sinai Hospital, Toronto (D.E. Low, R.G. Melano)

Acknowledgment

We thank Prasad Rawte, Stephen Lo, and Heather Siebert for providing technical support.

References

  1. Centers for Disease Control and Prevention. Update: detection of a Verona integron-encoded metallo-β-lactamase in Klebsiella pneumoniae—United States, 2010. MMWR Morb Mortal Wkly Rep. 2010;59:1212 .PubMed
  2. Giske CG, Gezelius L, Samuelsen Ø, Warner M, Sundsfjord A, Woodford N. A sensitive and specific phenotypic assay for detection of metallo-β-lactamases and KPC in Klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin. Clin Microbiol Infect. 2011;17:5526 . DOIPubMed
  3. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 20th Informational supplement M100–S20. Wayne (PA): The Institute; 2010.
  4. Tijet N, Alexander DC, Richardson D, Lastovetska O, Low DE, Patel SN, New Delhi metallo-β-lactamase, Ontario, Canada. Emerg Infect Dis. 2011;17:3067 . DOIPubMed
  5. Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods. 2005;63:21928. DOIPubMed
  6. Wirth T, Falush D, Lan R, Colles F, Mensa P, Wieler LH, Sex and virulence in Escherichia coli: an evolutionary perspective. Mol Microbiol. 2006;60:113651. DOIPubMed
  7. Rogers BA, Sidjabat HE, Paterson DL. Escherichia coli O25b-ST131: a pandemic, multiresistant, community-associated strain. J Antimicrob Chemother. 2011;66:114. DOIPubMed
  8. Mantengoli E, Luzzaro F, Pecile P, Cecconi D, Cavallo A, Attala L, Escherichia coli ST131 producing extended-spectrum β-lactamases plus VIM-1 carbapenemase: further narrowing of treatment options. Clin Infect Dis. 2011;52:6901. DOIPubMed
  9. Barton BM, Harding GP, Zuccarelli AJ. A general method for detecting and sizing large plasmids. Anal Biochem. 1995;226:23540. DOIPubMed

Table

Suggested citation for this article: Tijet N, Macmullin G, Lastovetska O, Vermeiren C, Wenzel P, Stacey-Works T, et al. Verona integron–encoded metallo-β-lactamase 1 in Enterobacteria, Ontario, Canada [letter]. Emerg Infect Dis [Internet]. 2013 Jul [date cited]. http://dx.doi.org/10.3201/eid1907.121294

DOI: 10.3201/eid1907.121294

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Table of Contents – Volume 19, Number 7—July 2013

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Roberto G. Melano, Ontario Agency for Health Protection and Promotion, Public Health Laboratory Branch, 81 Resources Rd, Toronto, Ontario M9P 3T1, Canada

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