Aichi Virus in Sewage and Surface Water, the Netherlands
Figure 1. . . Maximum-clade credibility tree showing the phylogenetic relationships between Aichi virus isolates from the Netherlands and other locations, based on a multiple alignment of nucleotide sequences (481-nt) of the viral protein (VP) 1 region. The rooted tree was generated by the Bayesian Markov chain Monte Carlo method in BEAST (28), using CK as an outgroup, visualized in FigTree (http://tree.bio.ed.ac.uk/software/figtree/), and plotted on a temporal y-axis scale in units of 1,000 years. Aichi virus strains from the Netherlands isolated from sewage (red) and surface waters (blue) are indicated; reference strains (black) are shown with GenBank accession numbers. Clusters of sequences of the same sample are represented by triangles (a collapsed branch), and the number of isolates in each triangle is shown in parentheses. Aichi virus genotypes are shown on the right (A, B, and C). CK, canine kobuviruses.