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Volume 19, Number 8—August 2013

Research

Aichi Virus in Sewage and Surface Water, the Netherlands

Willemijn J. LodderComments to Author , Saskia A. Rutjes, Katsuhisa Takumi, and Ana Maria de Roda Husman
Author affiliations: National Institute of Public Health and the Environment (RIVM), Bilthoven, the Netherlands (W.J. Lodder, S.A. Rutjes, K. Takumi, A.M. de Roda Husman); Utrecht University, Utrecht, the Netherlands (A.M. de Roda Husman).

Main Article

Table 1

Oligonucleotide sequences of the VP1 and VP3 primers developed and used for study of Aichi virus in sewage and surface water, the Netherlands*

Primer Sequence, 5′→3′† Nucleotide location‡ PCR
AiV-VP3-F1 CACACCGCCCCTGCGTCRGCCCTCGT 2912–2937 First-round
AiV-VP1-F2 CTCGATGCRCCMCAAGACACCGG 3023–3045 Nested
AiV-VP1-F3 GTGCTTCACRTACATCGCYGCGG 3289–3311 Nested
AiV-VP1-R2 CCTGACCAGTCCTCCCAWCCGAAGTA 3552–3527 Nested
AiV-VP1-R1 GAGAGCTGGAAGTCRAAGGG 3651–3632 First-round

*VP, viral protein; AiV, Aichi virus; F, forward; R, reverse.
†R indicates A or G; M indicates A or C; Y indicates C or T; W indicates A or T.
‡Compared with Aichi virus reference strain (GenBank accession no. AB010145).

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