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Volume 19, Number 9—September 2013

Research

Use of Staged Molecular Analysis to Determine Causes of Unexplained Central Nervous System Infections

Chien-Chin HsuComments to Author , Rafal Tokarz, Thomas Briese, Hung-Chin Tsai, Phenix-Lan Quan, and W. Ian Lipkin
Author affiliations: Columbia University, New York, New York, USA (C.-C. Hsu, R. Tokarz, T. Briese, P.-L. Quan, W.I. Lipkin); Southern Taiwan University of Science and Technology, Tainan, Taiwan (C.-C. Hsu); Chi-Mei Medical Center, Tainan (C.-C. Hsu); National Yang-Ming University Kaohsiung, Taiwan (H.-C. Tsai); Kaohsiung Veterans General Hospital, Kaohsiung (H.-C. Tsai)

Main Article

Table 3

Pathogens identified by a staged molecular approach

Pathogen No. (%) cases
No. HIV positive Molecular method
Total Meningitis Encephalitis
Virus
Enterovirus* 2 (6) 2 (8) 0 0 MassTag PCR
Human herpesvirus 1 2 (6) 0 2 (33) 0 MassTag PCR/microarray†
Human herpesvirus 2 1 (3) 1 (4) 0 0 MassTag PCR
Varicella-zoster virus 3 (10) 0 3 (50) 0 MassTag PCR
Epstein-Barr virus 16 (52) 16 (64) 0 9 MassTag PCR
Cytomegalovirus
1 (3)
0
1 (17)
0
MassTag PCR
Bacteria
Mycobacterium tuberculosis 1 (3) 1 (4) 0 0 MassTag PCR
Escherichia coli
5 (16)
5 (20)
0
1
16S rRNA PCR
Total 31 (100) 25 (100) 6 (100) 10

*Sequences of enteroviruses showed 1 infection each of echovirus 11 and echovirus 30.
†For 1 case, human herpesvirus 1 was identified by MassTag PCR; in the other, by DNA microarray.

Main Article

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