Two Morbilliviruses Implicated in Bottlenose Dolphin Epizootics
Figure. Partial nucleotide sequence of DMV and PMV morbillivirus P gene compared with published DMV and PMV sequences (12). Primer sequences used were 5'-ATC TGC TCC CAG GAT TAA GGT CGA-3' (forward) and 5'-CGG GAT TGG TGG GAC CTT TA-3' (reverse). RT- PCR was performed (11). PCR products were cycle sequenced (20) or cloned into PDK101 (21) and sequenced by using T7 Sequenase (Amersham Corporation, Arlington Heights, IL) according to the manufacturer’s instructions.