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Volume 20, Number 2—February 2014
Research

Novel Paramyxovirus Associated with Severe Acute Febrile Disease, South Sudan and Uganda, 2012

César G. Albariño, Michael Foltzer, Jonathan S. Towner, Lory A. Rowe, Shelley Campbell, Carlos M. Jaramillo, Brian H. Bird, DeeAnn M. Reeder, Megan E. Vodzak, Paul Rota, Maureen G. Metcalfe, Christina F. Spiropoulou, Barbara Knust, Joel P. Vincent, Michael A. Frace, Stuart T. Nichol, Pierre E. Rollin, and Ute StröherComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C.G. Albariño, J.S. Towner, L.A. Rowe, S. Campbell, B.H. Bird, P. Rota, M.G. Metcalfe, C.F. Spiropoulou, B. Knust, J.P. Vincent, M.A. Frace, S.T. Nichol, P.E. Rollin, U. Ströher); Geisinger Medical Center, Danville, Pennsylvania, USA (M. Foltzer, C.M. Jaramillo); Bucknell University, Lewisburg, Pennsylvania, USA (D.M. Reeder, M.E. Vodzak)

Main Article

Figure 2

A) Organization of the viral genome of novel paramyxovirus related to rubula-like viruses isolated from fruit bats was determined from the full-length sequence. B) Localization of the predicted viral genes and open reading frames (ORFs). The V/P edition site is predicted from the similarity to Tuhoko virus 3. C) Terminal sequences were determined by standard rapid amplification of cDNA ends (RACE) methods. The complementarity of terminal sequences is shown in vRNA and vcRNA sense. D) Amino acid

Figure 2. A) Organization of the viral genome of novel paramyxovirus related to rubula-like viruses isolated from fruit bats was determined from the full-length sequenceB) Localization of the predicted viral genes and open reading frames (ORFs)The V/P edition site is predicted from the similarity to Tuhoko virus 3C) Terminal sequences were determined by standard rapid amplification of cDNA ends (RACE) methodsThe complementarity of terminal sequences is shown in vRNA and vcRNA senseD) Amino acid sequences of the nucleocapsid (N) protein of 22 representative paramyxovirus sequences were aligned by using the MUSCLE algorithm (CLC Genomics Workbench version 6.0.1; CLC bio, Cambridge, MA, USA)The phylogenetic analysis was conducted with a Bayesian algorithm (Mr.Bayes, Geneious version 6.1.5, www.geneious.com/)NP sequences were extracted from the complete genomic sequences in GenBank: KF774436 (Sosuga virus [SosV]), GU128082 (Tuhoko virus 3 ), GU128081 (Tuhoko virus 2), GU128080 (Tuhoko virus 1), AF298895 (Tioman virus), NC_007620 (Menangle virus), JX051319 (Achimota virus 1), JX051320 (Achimota virus 2), NC_003443 (human parainfluenza virus type 2), AF052755 (simian parainfluenza virus 5), HQ660095 (bat paramyxovirus Epo_spe/AR1/DRC/2009), NC_002200 (mumps virus), NC_009489 (Mapuera virus), NC_009640 (porcine rubulavirus), NC_001498 (measles virus), NC_006296 (rinderpest virus), NC_001921 (canine distemper virus), NC_001552 (Sendai virus), NC_003461 (human parainfluenza virus type 1), NC_002728 (Nipah virus), NC_001906 (Henra virus), NC_002617 (Newcastle disease virus)vcRNA, viral complementary RNA; N, nucleocapsid protein; V/P, V protein; M, matrix protein; F, fusion protein; HN, hemagglutinin-neuraminidase; L, molecular weight DNA ladder; CDS, coding sequence; nt posnucleotide position; vRNA, viral RNA.

Main Article

Page created: January 15, 2014
Page updated: January 15, 2014
Page reviewed: January 15, 2014
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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