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Volume 20, Number 4—April 2014

Dispatch

Genetic Characterization of Clade 2.3.2.1 Avian Influenza A(H5N1) Viruses, Indonesia, 2012

Ni Luh Putu Indi DharmayantiComments to Author , Risza Hartawan, Pudjiatmoko, Hendra Wibawa, Hardiman, Amanda Balish, Ruben Donis, C. Todd Davis, and Gina Samaan
Author affiliations: Indonesian Research Center for Veterinary Science, Bogor, Indonesia (N.L.P.I. Dharmayanti, R. Hartawan, Hardiman); Ministry of Agriculture, Jakarta, Indonesia (Pudjiatmoko); Disease Investigation Center Wates, Jogjakarta, Indonesia (H. Wibawa); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (A. Balish, R. Donis, C.T. Davis); Centers for Disease Control and Prevention, Jakarta (G. Samaan)

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Table 2

Hemagglutination-inhibition assay of clade 2.3.2.1 highly pathogenic avian influenza A(H5N1) virus introduced into Indonesia, 2012*

Antigen† Clade Reference ferret antiserum
1
2.2.1
2.3.4
2.1.3.2
2.3.2.1
2.3.2.1
2.3.2.1
2.3.2.1
2.3.2.1
VN/1203 EG/321 ANH/1 IND/ 12379 CH/1 RG30 BS/HK/ 1161 BHG/MG/X53 HK/ 6841 DK/VN/ 1584
Reference strains
VN/1203 1 320* 20 40 <10 10 <10 10 10 10
EG/321 2.2.1 80 1,280 80 20 40 10 80 80 40
ANH/1 2.3.4 160 80 640 80 <10 <10 <10 40 10
IND/12379 2.1.3.2 10 10 40 1,280 <10 <10 <10 40 40
CH/1 RG30 2.3.2.1 40 80 20 20 640 40 160 640 160
BS/HK/1161 2.3.2.1 <10 40 10 <10 320 80 160 640 80
BHG/MG/X53 2.3.2.1 10 80 20 20 320 40 320 640 320
HK/6841 2.3.2.1 10 20 10 20 160 20 320 640 160
DK/VN/1584
2.3.2.1
<10
40
10
20
320
20
320
320
160
Test strain
A/environment/East 
 Java/LBM-LM13/2012 2.3.2.1 <10 40 10 10 160 40 320 320 160

*Homologous titers of reference antigen to ferret antiserum are indicated in boldface.
†Strains: VN/1203, A/Vietnam/1203/2004; EG/321, A/Egypt/2321-NAMRU3/2007; ANH/1, A/Anhui/1/2005; IND/12379, A/Indonesia/NIHRD12379/2012; CH/1 RG30, A/Hubei/1/2010 IDCDC-RG30; BS/HK/1161, A/barn swallow/Hong Kong/1161/2010; BHG/MG/X53, A/barheaded goose/Mongolia/X53/2009; HK/6841, A/Hong Kong/6841/2010; DK/VN/1584, A/duck/Vietnam/NCVD-1584/2012.

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