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Volume 20, Number 4—April 2014

Dispatch

Pathology of US Porcine Epidemic Diarrhea Virus Strain PC21A in Gnotobiotic Pigs

Kwonil Jung, Qiuhong Wang1Comments to Author , Kelly A. Scheuer, Zhongyan Lu, Yan Zhang, and Linda J. Saif1
Author affiliations: The Ohio State University, Wooster, Ohio, USA (K. Jung, Q. Wang, K.A. Scheuer, Z. Lu, L.J. Saif); Ohio Department of Agriculture, Reynoldsburg, Ohio, USA (Y. Zhang)

Main Article

Table 1

Design and results of a study of the pathology of US PEDV strain PC21A in gnotobiotic pigs, 2013*

Pig status, pig no.; age, d, at inoculation Inoculum passage no. Intranasal/oral inoculum, log10 GE Onset of clinical signs, PIH Viral RNA in serum sample at euthanasia, log10 GE/mL†
Fecal shedding, log10 GE/mL, by PIH
0 24 48 72 96
PEDV-inoculated
1; 10 1 7.3/7.7 <4.8† 10.7‡ 25–26 7.6
2; 18 2 6.3/6.8 <4.8 <4.8 11.0 11.2‡ 44–46 6.3
3; 24 3 8.3/8.8 <4.8 10.2 12.3‡ 44–46 5.7
4; 24§ 4 Indirect contact <4.8 10.9 9.6 10.6 ND 2–4§ 7.6
5; 35
4
0/9.0
<4.8
ND
ND


26–30
4.8
Negative control
6; 26 . . <4.8 <4.8 <4.8 <4.8 <4.8 . <3.8†

*PEDV, porcine epidemic diarrhea virus; GE, genome equivalents; PIH, postinoculation hour; –, no result (pig euthanized); ND, not determined.
†Detected by real-time reverse transcription PCR with a detection limit of 4.8 log10 GE/mL for fecal samples and 3.8 log10 GE/mL for serum samples.
‡Euthanized.
§At 24 days of age, noninoculated pig 4 was exposed by indirect contact to pig 3 (at PIH 0) through small holes drilled into the stainless steel divider panel located between the 2 pigs in the shared pig tub isolator unit. Clinical signs and virus shedding were monitored after indirect contact. Diarrhea and vomiting developed in pig 4 approximately 2–4 h after clinical signs developed in pig 3 (i.e., in pig 4, signs developed 46–50 h after indirect contact with inoculated pig 3); pig 4 was euthanized ≈120 h after the onset of clinical signs.

Main Article

1These authors were co-principal investigators.

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