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Volume 20, Number 4—April 2014

Dispatch

Pathology of US Porcine Epidemic Diarrhea Virus Strain PC21A in Gnotobiotic Pigs

Kwonil Jung, Qiuhong Wang1Comments to Author , Kelly A. Scheuer, Zhongyan Lu, Yan Zhang, and Linda J. Saif1
Author affiliations: The Ohio State University, Wooster, Ohio, USA (K. Jung, Q. Wang, K.A. Scheuer, Z. Lu, L.J. Saif); Ohio Department of Agriculture, Reynoldsburg, Ohio, USA (Y. Zhang)

Main Article

Table 2

Histopathologic findings in a study of the pathology of US PEDV strain PC21A in gnotobiotic pigs, 2013*

Pig status, no. PIH at euthanasia (infection stage) VH:CD, mean (±SD) Antigen detection in frozen tissues†
Duodenum Jejunum Ileum Cecum/colon Lung
PEDV-inoculated
1 30 (acute) 3.4 (1.7) ++ +++ +++ +++
2 72 (mid) 1.8 (0.3) ++ +++ +++ +++
3 46 (acute) 1.2 (0.3) + ++ ++ ++
4‡ 120 (later)‡ 1.5 (0.2) + +/++ +/++ ++/+++
5
67 (mid)
2.2 (0.4)
+
+++
+++
+

Negative control
6 . 6.3 (0.7)

*PEDV, porcine epidemic diarrhea virus; PIH, postinoculation hour; VH:CD, ratio of villous height to crypt depth.
†Detected by immunofluorescence staining. +, 1%–29% of epithelial cells showed staining; ++, 30%–59% of epithelial cells showed staining; +++, 60%–100% of epithelial cells showed staining, as described (4); –, no cells showed staining.
‡At 24 days of age, noninoculated pig 4 was exposed by indirect contact to pig 3 (at PIH 0) through small holes drilled into the stainless steel divider panel located between the 2 pigs in the shared pig tub isolator unit. Clinical signs and virus shedding were monitored after indirect contact. Diarrhea and vomiting developed in pig 4 approximately 2–4 h after clinical signs developed in pig 3 (i.e., in pig 4, signs developed 46–50 h after indirect contact with inoculated pig 3); pig 4 was euthanized ≈120 h after the onset of clinical signs.

Main Article

1These authors were co-principal investigators.

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