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Volume 20, Number 4—April 2014

Research

Antibodies against MERS Coronavirus in Dromedary Camels, United Arab Emirates, 2003 and 2013

Benjamin Meyer, Marcel A. Müller, Victor M. Corman, Chantal B.E.M. Reusken, Daniel Ritz, Gert-Jan Godeke, Erik Lattwein, Stephan Kallies, Artem Siemens, Janko van Beek, Jan F. Drexler, Doreen Muth, Berend-Jan Bosch, Ulrich Wernery, Marion P.G. Koopmans, Renate Wernery, and Christian DrostenComments to Author 
Author affiliations: University of Bonn Medical Centre, Bonn, Germany (B. Meyer, M.A. Müller, V.M. Corman, D. Ritz, S. Kallies, A. Siemens, J.F. Drexler, D. Muth, C. Drosten); National Institute for Public Health and the Environment, Bilthoven, the Netherlands. (C.B.E.M. Reusken, G.-J. Godeke, J. van Beek, M.P.G. Koopmans); Erasmus Medical Centre, Rotterdam, the Netherlands (C.B.E.M. Reusken, J.F. Drexler, M.P.G. Koopmans); EUROIMMUN AG, Lübeck, Germany (E. Lattwein); Utrecht University, Utrecht, the Netherlands (B.-J. Bosch); Central Veterinary Research Laboratory, Dubai, United Arab Emirates (U. Wernery, R. Wernery)

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Figure

Immunofluorescence and microarray reactivity patterns for antibodies (SF74, H40, H97, and S11) against Middle East respiratory syndrome coronavirus (MERS-CoV) in serum samples from camels, United Arab Emirates, 2003 and 2012. A) Serum samples tested against overexpressed MERS-CoV spike protein (MERS-S), overexpressed human CoV-OC43 spike protein (OC43-S), and Vero cells infected with MERS-CoV (MERS-V). Fluorescence intensities were evaluated as follows: –, negative; +, weakly reactive; ++, react

Figure. . . Immunofluorescence and microarray reactivity patterns for antibodies (SF74, H40, H97, and S11) against Middle East respiratory syndrome coronavirus (MERS-CoV) in serum samples from camels, United Arab Emirates, 2013. A) Serum samples tested against overexpressed MERS-CoV spike protein (MERS-S), overexpressed human CoV-OC43 spike protein (OC43-S), and Vero cells infected with MERS-CoV (MERS-V). Fluorescence intensities were evaluated as follows: –, negative; +, weakly reactive; ++, reactive; +++, strongly reactive. Scale bar indicates 20 μm. B) Relative fluorescence units (RFU) were determined for the same serum samples by microarray using S1 domains of MERS-CoV and human CoV-OC43.

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