Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 20, Number 5—May 2014

Research

Molecular Investigation of Tularemia Outbreaks, Spain, 1997–2008

Jaime Ariza-Miguel, Anders Johansson, María Isabel Fernández-Natal, Carmen Martínez-Nistal, Antonio Orduña, Elías F. Rodríguez-Ferri, Marta Hernández, and David Rodríguez-LázaroComments to Author 
Author affiliations: Instituto Tecnológico Agrario de Castilla y León, Valladolid, Spain (J. Ariza-Miguel, M. Hernández, D. Rodríguez-Lázaro); Umeå University, Umeå, Sweden (A. Johansson); Complejo Asistencial Universitario de León, León, Spain (M.I. Fernández-Natal); Laboratorio Regional de Sanidad Animal León, Valladolid (C. Martínez-Nistal); Universidad de Valladolid, Valladolid (A. Orduña); Universidad de León, León (E.F. Rodríguez-Ferri); Universidad de Burgos, Burgos, Spain (D. Rodríguez-Lázaro)

Main Article

Table

Multilocus variable number tandem repeat analysis of 98 Francisella tularensis isolates from Spain and 11 reference isolates*

MLVA genotype No. isolates (origin) No. MLVA markers that discriminated F. tularensis subsp. holarctica isolates
Ft-M3 Ft-M6 Ft-M9 Ft-M20 Ft-M24†
A 49 (Spain) 5 4 2 3 2 (∆16 bp)
B 32 (Spain) 4 4 2 3 2 (∆16 bp)
C 9 (Spain) 6 4 2 3 2 (∆16 bp)
D 3 (Spain) 3 4 2 3 2 (∆16 bp)
E 2 (Spain) 4 7 2 3 2 (∆16 bp)
F 1 (Spain) 4 4 2 4 2 (∆16 bp)
G 1 (Spain) 4 4 3 3 2 (∆16 bp)
H 1 (Spain) 7 4 2 3 2 (∆16 bp)
I 4 (former Czechoslovakia) 4 6 2 3 2
J 2 (Czech Republic) 5 7 2 3 2
K 2 (Czech Republic) 6 7 2 3 2
L 1 (former Czechoslovakia) 7 4 2 2 2
M 1 (Czech Republic) 7 7 2 3 2
N‡ 1 (United States) 28 4 4 3 1

*MLVA, multilocus variable number tandem repeat.
†All isolates from Spain had the unique 16-bp deletion at marker FT-M24 that is characteristic of genetic subclade B.Br:FTNF002–00 (Iberian clone).
‡Genotype N, F. tularensis subsp. tularensis strain Schu, showed additional unique alleles at the following MLVA markers: Ft-M5, Ft-M7, Ft-M8, Ft-M10, Ft-M13, Ft-M16, Ft-M19, Ft-M21, Ft-M22, and Ft-M23.

Main Article

TOP