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Volume 20, Number 5—May 2014

Dispatch

PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus

Wei Wang1, Zhigang Song1, Wencai Guan1, Yi Liu, Xiaonan Zhang, Lei Xu, Jianhua Li, Zhenghong Yuan, and Yunwen HuComments to Author 
Author affiliations: Shanghai Public Health Clinical Center, Shanghai, China

Main Article

Figure

Dynamic range of reverse transcription PCR for detection of oseltamivir resistance in influenza A(H7N9) virus. Amplification curves (ΔRn vs. cycle number) for serial dilutions of plasmid with 292K (mutant) or R292 (wild-type) neuraminidase (NA) fragments. ΔRN is change in signal magnitude (reporter signal minus baseline signal). Assay dynamic range was linear at template concentrations of 102–108 copies/reaction. A) Detection of NA 292K mutant strain with probe N9-K: slope = −3.388, R2 = 0.997.

Figure. Dynamic range of reverse transcription PCR for detection of oseltamivir resistance in influenza A(H7N9) virusAmplification curves (ΔRn vscycle number) for serial dilutions of plasmid with 292K (mutant) or R292 (wild-type) neuraminidase (NA) fragmentsΔRN is change in signal magnitude (reporter signal minus baseline signal)Assay dynamic range was linear at template concentrations of 102–108 copies/reactionA) Detection of NA 292K mutant strain with probe N9-K: slope = −3.388, R2 = 0.997Light and dark blue curves indicate probe NA 292K in duplicate wellsViolet curves indicate control wellsB) Detection of NA R292 wild-type strain with probe N9-R: slope = −3.672, R2 = 0.992Different colored curves indicate probe N9-R in triplicate wells.

Main Article

1These authors contributed equally to this article.

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