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Volume 20, Number 5—May 2014

Dispatch

PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus

Wei Wang1, Zhigang Song1, Wencai Guan1, Yi Liu, Xiaonan Zhang, Lei Xu, Jianhua Li, Zhenghong Yuan, and Yunwen HuComments to Author 
Author affiliations: Shanghai Public Health Clinical Center, Shanghai, China

Main Article

Table

Detection of wild-type neuraminidase R292 and mutant 292K influenza A(H7N9) virus in clinical samples from 9 patients in China, by reverse transcription PCR*

Patient no.† Outcome Sample type Time after oseltamivir treatment started, d Time after oseltamivir treatment ended, d 292K:R292 ratio Viral load, copies/mL‡
2 Died NPS 9 −1 100:0 1.78 × 105
S 9 −1 100:0 8.76 × 104
NPS 10 −2 100:0 6.14 × 104


S
10
−2
25.7:74.3
1.29 × 104
3 Died NPS 13 3 ND
S 13 3 3.03 × 103
NPS 16 6 ND


S
16
6
100:0
1.21 × 104
5 Died NPS 7 −4 9.96 × 102


S
11
0

ND
6 Recovered NPS 16 −3 ND


S
19
0

ND
7 Recovered NPS 13 0 ND


S
13
0

ND
8 Recovered NPS 7 0 ND


S
7
0
100:0
1.76 × 104
10 Recovered NPS 11 −4 ND


S
15
0
91.7:8.3
5.25 × 104
15 Died NPS 20 4 ND


S
20
4

5.62 × 103
17 Recovered NPS 11 0 ND
S 11 0 3.06 × 104

*NPS, nasopharyngeal swab specimen; S, sputum specimen; ­–, no ratio because samples had negative results; ND, not detected.
†Patient identification was identical with that used by Hu et al. (3). Patients 15 and 17 are initially reported in the current study.
‡Method used to determine viral load was reported by Hu et al. (3).

Main Article

1These authors contributed equally to this article.

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