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Volume 20, Number 7—July 2014
Dispatch

Detection and Genetic Characterization of Deltacoronavirus in Pigs, Ohio, USA, 2014

Leyi Wang1, Beverly Byrum, and Yan Zhang1Comments to Author 
Author affiliations: Ohio Department of Agriculture, Reynoldsburg, Ohio, USA

Main Article

Table 2

Oligonucleotide primers used for amplification of the porcine coronavirus HKU15 genomic fragments by reverse transcription PCR, Ohio, USA, 2014

Primer identification Sequence (5′→3′) Nucleotide position* Fragment
Dcor-1-F ACATGGGGACTAAAGATAAAAATTATAGC 1–29 1
DCor-1610-R
AGACGGGCCAATTTTGACCG
1591–1610
DCor-1481-F TGATGATGTT CTGCTAGCCT 1481–1500 2
DCor-3300-R
GCTCATCGCCTACATCAGTA
3281–3300
DCor-3091-F CGGATTTAAAACCACAGACT 3091–3110 3
Dcor-4860-R
ACGACTTTACGAGGATGAAT
4841–4860
DCor-4741-F CTCCTGTACAGGCCTTACAA 4741–4760 4
DCor-6420-R
TCACACGTATAGCCTGCTGA
6401–6420
DCor-6291-F CTCAATGCAGAAGACCAGTC 6291–6310 5
DCor-8041-R
CAGCTTGGTCTTAAGACTCT
8041–8060
DCor-7920-F GGTACTGCTTCTGATAAGGAT 7920–7940 6
DCor-9660-R
TAGGTACAGTTGTGAACCGA
9641–9660
DCor-9541-F CTCTGCCCATTATCATGCCT 9541–9560 7
DCor-11040-R
AAAGAGAGGCATTTTGCTGG
11021–11040
DCor-10861-F ACTTGGACCCTCCTATGCGC 10861–10880 8
DCor-12840-R
GGCTCAAGATACTTATCTGC
12821–12840
DCor-12721-F TATGCAGGATGGTGAAGCGG 12721–12740 9
DCor-14400-R
TCACAATAAATCGCAGTGCC
14381–14000
DCor-14281-F TGTTACGCAGACTACACATA 14281–14300 10
DCor-16020-R
TCATAGCCGCAGCGCTTAAA
16001–16020
DCor-15901-F TGTGGTGTTTAGGCAGGCAA 15901–15920 11
DCor-17760-R
GTGGCGGTTACGCCTAAACC
17741–17760
DCor-17641-F CAAACTCTTTGACAATCGCA 17641–17660 12
DCor-19200-R
GCTAAAGGAGAATAGGTTGGTG
19179–19200
DCor-18981-F CTGAACATTCATTCTCACCC 18981–19000 13
DCor-20910-R
GAAGGTGGTGGCATTTGTGG
20891–20910
DCor-20761-F GTCTTACCGTGTGAAACCCC 20761–20780 14
DCor-22440-R
AACATCCCACTGAGGAGGTG
22421–22440
DCor-22321-F TTTTATAACACCACCGCTGC 22321–22340 15
DCor-24004-R
GGCCATGATAGATTGGTGTC
23985–24004
DCor-23881-F ATGGTGAGCCTTTACTGCTT 23881–23900 16
DCor-25417-R TGCTCCATCCCCCCTATAAG 25398–25417

*Positions correspond to porcine coronavirus HKU15-155 strain (GenBank accession no. JQ065043).

Main Article

1These authors were co–principal investigators.

Page created: June 17, 2014
Page updated: June 17, 2014
Page reviewed: June 17, 2014
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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