TY - JOUR AU - Etienne, Kizee AU - Roe, Chandler AU - Smith, Rachel AU - Vallabhaneni, Snigdha AU - Duarte, Carolina AU - Escandón, Patricia AU - Castañeda, Elizabeth AU - Gómez, Beatriz AU - de Bedout, Catalina AU - López, Luisa AU - Salas, Valentina AU - Hederra, Luz Maria AU - Fernández, Jorge AU - Pidal, Paola AU - Hormazabel, Juan Carlos AU - Otaíza-O’Ryan, Fernando AU - Vannberg, Fredrik AU - Gillece, John AU - Lemmer, Darrin AU - Driebe, Elizabeth AU - Engelthaler, David AU - Litvintseva, Anastasia T1 - Whole-Genome Sequencing to Determine Origin of Multinational Outbreak of Sarocladium kiliense Bloodstream Infections T2 - Emerging Infectious Disease journal PY - 2016 VL - 22 IS - 3 SP - 476 SN - 1080-6059 AB - We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013–2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (<5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures. KW - fungal outbreaks KW - epidemiology KW - whole-genome sequencing KW - fungi KW - Latin America KW - bloodstream infections KW - Chile KW - Colombia DO - 10.3201/eid2203.151193 UR - https://wwwnc.cdc.gov/eid/article/22/3/15-1193_article ER - End of Reference