TY - JOUR AU - Cordey, Samuel AU - Vu, Diem-Lan AU - Schibler, Manuel AU - L’Huillier, Arnaud AU - Brito, Francisco AU - Docquier, Mylène AU - Posfay-Barbe, Klara AU - Petty, Thomas AU - Turin, Lara AU - Zdobnov, Evgeny AU - Kaiser, Laurent T1 - Astrovirus MLB2, a New Gastroenteric Virus Associated with Meningitis and Disseminated Infection T2 - Emerging Infectious Disease journal PY - 2016 VL - 22 IS - 5 SP - 846 SN - 1080-6059 AB - Next-generation sequencing has identified novel astroviruses for which a pathogenic role is not clearly defined. We identified astrovirus MLB2 infection in an immunocompetent case-patient and an immunocompromised patient who experienced diverse clinical manifestations, notably, meningitis and disseminated infection. The initial case-patient was identified by next-generation sequencing, which revealed astrovirus MLB2 RNA in cerebrospinal fluid, plasma, urine, and anal swab specimens. We then used specific real-time reverse transcription PCR to screen 943 fecal and 424 cerebrospinal fluid samples from hospitalized patients and identified a second case of meningitis, with positive results for the agent in the patient’s feces and plasma. This screening revealed 5 additional positive fecal samples: 1 from an infant with acute diarrhea and 4 from children who had received transplants. Our findings demonstrate that astrovirus MLB2, which is highly prevalent in feces, can disseminate outside the digestive tract and is an unrecognized cause of central nervous system infection. KW - high-throughput nucleotide sequencing KW - mamastrovirus KW - viral meningitis KW - viremia KW - immunocompromised host KW - viruses KW - astrovirus KW - astrovirus MLB2 KW - enteric infections DO - 10.3201/eid2205.151807 UR - https://wwwnc.cdc.gov/eid/article/22/5/15-1807_article ER - End of Reference