Figure 2. Direct fluorescent staining of the frozen sections of midguts of X. cheopis fleas showing R. typhi-infected epithelial cells at 3 (A) and 10 days (B) postinfectious feeding. Fleas were embedded individually in OCT compound (Miles Laboratories, Naperville, IL), sectioned (4-6 m)(16), and stained with fluorescein isothiocyanate (FITC)-labeled guinea pig anti-R. typhi IgG. G: gut lumen.
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