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Volume 3, Number 4—December 1997
THEME ISSUE
Foodborne

Controlling Emerging Foodborne Microbial Hazards

Epidemiology and Detection as Options for Control of Viral and Parasitic Foodborne Disease

Lee-Ann JaykusComments to Author 
Author affiliation: North Carolina State University, Raleigh, North Carolina, USA

Main Article

Table 3

Emerging detection methods for parasitic protozoa in water

Pathogen Detection limit Viability Differentiation Ref.
Flow Cytometry with Fluorescent Imaging and CCD
Cryptosporidium NR Yes, using differential fluorogenic vital dyes NR 58,59
DNA Hybridization
Giardia
(16s-like rDNA) 1cyst NR No 60
Giardia
(16s-like rDNA) NR NR Yes-in situ hybridization with differential fluorescence
G. lamblia; G. muris 61
Polymerase Chain Reaction
Giardia
(Giardin gene) 1 cyst Partial, using mRNA
as target; Depends on
inactivation method NR 62
Giardia
(Giardin gene) 1 cyst NR Yes-primer sequence
G. duodenalis 63
Cryptosporidium
(Target not specified) 20 oocysts No Yes-by hybridization
C. parvum 64

CCD = cooled charge couple device; NR = not reported

Main Article

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