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Volume 3, Number 4—December 1997

Dispatch

Vero Cytotoxin-Producing Escherichia coli O157 Outbreaks in England and Wales, 1995: Phenotypic Methods and Genotypic Subtyping

Geraldine A. Willshaw*, Henry R. Smith*, Thomas Cheasty*, Patrick G. Wall†, and Bernard Rowe*
Author affiliations: *Central Public Health Laboratory, London, United Kingdom; †Communicable Disease Surveillance Centre, London, United Kingdom

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Figure

Pulsed-field gel electrophoresis of XbaI-digested genomic DNA of O157 VTEC PT2 isolated from outbreaks in 1995. Digests were separated on 1% agarose for 42h at a voltage gradient of 5.6 volts per cm with a pulse ramp time of 5 to 50 sec. Lane 1 contained a phage lambda DNA 48.5 kb ladder (Sigma). Lanes 2 to 8 contained digests of PT2 strains from outbreaks as follows: lane2, outbreak 1; lane3, outbreak 2; lane4, outbreak 4; lane5, outbreak 5; lane6, outbreak 6; lane7, outbreak 8; lane8, outbreak

Figure. Pulsed-field gel electrophoresis of XbaI-digested genomic DNA of O157 VTEC PT2 isolated from outbreaks in 1995. Digests were separated on 1% agarose for 42h at a voltage gradient of 5.6 volts per cm with a pulse ramp time of 5 to 50 sec. Lane 1 contained a phage lambda DNA 48.5 kb ladder (Sigma). Lanes 2 to 8 contained digests of PT2 strains from outbreaks as follows: lane2, outbreak 1; lane3, outbreak 2; lane4, outbreak 4; lane5, outbreak 5; lane6, outbreak 6; lane7, outbreak 8; lane8, outbreak 9.

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