Differentiating Human from Animal Isolates of Cryptosporidium parvum
Figure. Human- and bovine-specific restriction enzymes showed distinct banding pattern for genotypes of Cryptosporidium parvum isolates. The different lanes represent the TRAP-C2 PCR-amplified products belonging to AGA43, AMD36, AOH6, HM3, and HM5 isolates of C. parvum, respectively, after digestion with HaeIII (Lanes 1-5, human-specific marker) and BstE II (Lanes 7-11, bovine-specific marker) restriction enzymes and agarose gel electrophoresis. Lane 6 is the 100 bp marker. Samples AGA43, AMD36 and AOH6 are bovine (bovine genotype) and samples HM3 and HM5 are human (human genotype). Human- or bovine-specific restriction enzyme markers can cut only the TRAP-C2 amplified product of the respective genotype of C. parvum isolates.