Emerging Infectious Disease ISSN: 1080-6059

Differentiating Human from Animal Isolates of Cryptosporidium parvum

Irshad M. Sulaiman, Lihua Xiao, Chunfu Yang, Lilian Escalante, Anne Moore, Charles B. Beard, Michael J. Arrowood, and Altaf A. Lal

Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA

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Figure

Human- and bovine-specific restriction enzymes showed distinct banding pattern for genotypes of Cryptosporidium parvum isolates. The different lanes represent the TRAP-C2 PCR-amplified products belonging to AGA43, AMD36, AOH6, HM3, and HM5 isolates of C. parvum, respectively, after digestion with HaeIII (Lanes 1-5, human-specific marker) and BstE II (Lanes 7-11, bovine-specific marker) restriction enzymes and agarose gel electrophoresis. Lane 6 is the 100 bp marker. Samples AGA43, AMD36 and AOH6

Figure. Human- and bovine-specific restriction enzymes showed distinct banding pattern for genotypes of Cryptosporidium parvum isolates. The different lanes represent the TRAP-C2 PCR-amplified products belonging to AGA43, AMD36, AOH6, HM3, and HM5 isolates of C. parvum, respectively, after digestion with HaeIII (Lanes 1-5, human-specific marker) and BstE II (Lanes 7-11, bovine-specific marker) restriction enzymes and agarose gel electrophoresis. Lane 6 is the 100 bp marker. Samples AGA43, AMD36 and AOH6 are bovine (bovine genotype) and samples HM3 and HM5 are human (human genotype). Human- or bovine-specific restriction enzyme markers can cut only the TRAP-C2 amplified product of the respective genotype of C. parvum isolates.

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