Mycoplasma penetrans Bacteremia and Primary Antiphospholipid Syndrome1
Figure 1. Polymerase chain reaction (PCR) detection of Mycoplasma penetrans in clinical samples. A. M. penetrans PCR genomic amplification with the primers MYCPENET-P and MYCPENET-N (7) and analyzed by electrophoresis in 2% agarose gel. Lysates from the following original samples: throat swab (lane 1); tracheal aspirate (lane 2); blood (lane 3); first blood subculture (HF-1 isolate) (lane 4); M. penetrans GTU-54-6A1 (lane 5), showing the amplification product of 407-bp; and negative control (lane 6). B. Southern blotting of the same material. Hybridization with the internal oligonucleotide (MYCPENET-S) probe confirmed the specific amplification of M. penetrans genetic sequences.