Volume 5, Number 5—October 1999
Cryptosporidium parvum in Oysters from Commercial Harvesting Sites in the Chesapeake Bay
|Site||Location||Bay location or river system||Fall 1997
|IFA||PCRa||Mice infectivity||IFA||PCR||Mice infectivity||IFA||Cp11||Mice infectivity||Waterb|
|C||Halfway Mark||Fishing Bay||29||HT||Neg||0||BT & HT||Neg||1||Pos||Neg||ND|
|F||Back Cove||Tangier Sound||ND||ND||ND||2||BT||Neg||6||ND||Neg||8|
|G||Old Woman's Leg||Tangier Sound||ND||ND||ND||0||BT||Neg||0||ND||Neg||ND|
aPolymerase chain reaction and restriction fragment-length polymorphism (PCR-RFLP) on small subunit rRNA gene, 18s.
bNumber of oocysts recovered per liter of filtered bay water.
cNumber of oysters found positive for oocysts out of 30 oysters examined from each site.
dNeg indicates that PCR using Cp11 primers failed to detect Cryptosporidium DNA in the DNA extracted from the ilea of mice that were intubated with pooled hemolymph and gill washings from oysters.
HT, human genotype; BT, bovine genotype; ND, not done; IFA, immunofluorescent assay; PCR, polymerase chain reaction.
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