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Volume 6, Number 5—October 2000

Research

Toxin Gene Expression by Shiga Toxin-Producing Escherichia coli: the Role of Antibiotics and the Bacterial SOS Response

Patrick T. Kimmitt, Colin R. Harwood, and Michael R. BarerComments to Author 

Author affiliations: The Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom

Article in Chinese

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Table

Induction of stx2 expression by antibiotics under different incubation conditionsa

Target of action Incubation conditionsb
Agent (disc content mg/L) mO2/37 O2/37 mO2/42 AnO2/37 O2/30 O2/42 Zone patternc
Background + - - - + - NA
DNA Gyrase
Quinolonesd + + + + + +/- >MIC
Novobiocin (30) + - - - + - >MIC
Folate metabolism
Trimethoprim (5) + + - + + - >MIC
Smx (25) + + - + - - >MIC
DNA
Metronidazole (50) - - - - - - NA
Furazolidone (50) + + - - + - =MIC
Cell envelope
Cephalexin (30) + + - - +/- - =MIC
Amoxycillin (25) + + - - - - =MIC
Amoxy/Clav (20/10) +/- - - - - - =MIC
Ampicillin (10) + + - - - - =MIC
Pip/Taz (10/75) + + - - - - =MIC
Imipenem (10) - - - - - - NA
Aztreonam (30) + + - - - - =MIC
Cefuroxime (5) + + - - - - =MIC
Ceftazidime (30) + + - - - - =MIC
Cefotaxime (30) + + - - - - =MIC
Fosfomycin (200) - - - - - - NA
Polymyxin B (300 IU) +/- - - - + - =MIC
Translation
Gentamicin (10) - - - - - - NA
Chloramphenicol (30) - - - - - - NA
Doxycycline (30) - - - - - - NA
Erythromycin (15) - - - - - - NA
Transcription
Rifampin (25) - - +/- - - - NA

aStx2 induction effects (zones of blue coloration, Figure 1): -, not detected, +/-, borderline induction, +, definite induction, >MIC, zone of induction within the zone of growth inhibition; =MIC, induction on the edge of the zone of inhibition; NA, not applicable; Amoxy/Clav, amoxycillin/clavulanic acid; Pip/Taz, piperacillin/tazobactam; Smx, sulphamethoxazole.
bO2, aerobic; mO2 microaerobic; AO2, anaerobic/incubation temperature °C.
cNote: The patterns indicated were detected in three separate experiments and recorded by two independent observers.
dThe quinolones tested are listed in the Materials and Methods section.

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