TY - JOUR AU - Cummings, Craig A. AU - Relman, David A. T1 - Using DNA Microarrays to Study Host-Microbe Interactions T2 - Emerging Infectious Disease journal PY - 2000 VL - 6 IS - 5 SP - 513 SN - 1080-6059 AB - Complete genomic sequences of microbial pathogens and hosts offer sophisticated new strategies for studying host-pathogen interactions. DNA microarrays exploit primary sequence data to measure transcript levels and detect sequence polymorphisms, for every gene, simultaneously. The design and construction of a DNA microarray for any given microbial genome are straightforward. By monitoring microbial gene expression, one can predict the functions of uncharacterized genes, probe the physiologic adaptations made under various environmental conditions, identify virulence-associated genes, and test the effects of drugs. Similarly, by using host gene microarrays, one can explore host response at the level of gene expression and provide a molecular description of the events that follow infection. Host profiling might also identify gene expression signatures unique for each pathogen, thus providing a novel tool for diagnosis, prognosis, and clinical management of infectious disease. KW - microarray KW - DNA KW - Host-Microbe Interactions KW - Genomics KW - genome sequence KW - United States DO - 10.3201/eid0605.000511 UR - https://wwwnc.cdc.gov/eid/article/6/5/00-0511_article ER - End of Reference