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Volume 6, Number 6—December 2000

Research

Hantavirus Pulmonary Syndrome Associated with Monongahela Virus, Pennsylvania

Luther V. Rhodes*Comments to Author , Cinnia Huang†, Angela J. Sanchez§, Stuart T. Nichol§, Sherif R. Zaki§, Thomas G. Ksiazek§, J.G. Humphreys¶, James J. Freeman*, and Kenneth R. Knecht*
Author affiliations: *Lehigh Valley Hospital, Allentown, Pennsylvania, USA; †New York State Department of Health, Albany, New York, USA; §Centers for Disease Control and Prevention, Atlanta, Georgia, USA; ¶Indiana University of Pennsylvania, Indiana, Pennsylvania, USA

Main Article

Figure 1

Pennsylvania county map highlighting the four counties involved (Potter, Schuylkill, Lehigh, and Berks) in the environmental investigations of Patient 1, and the single county of residence (Monroe) and rodent exposure of Patient 2.

Figure 1. Pennsylvania county map highlighting the four counties involved (Potter, Schuylkill, Lehigh, and Berks) in the environmental investigations of Patient 1, and the single county of residence (Monroe) and rodent exposure of Patient 2.

Main Article

1Three fragments of the virus genome were amplified by nested polymerase chain reaction to allow generation of nucleotide (nt) sequences 393 nt in length for an S segment region encoding the N protein, 259 nt in length for an M segment region (M-G1) encoding the G1 protein, and 205 nt in length for an M segment region (M-G2) encoding the G2 protein (12). Sequence data were analyzed by using the Wisconsin GCG Package (version 9.1) software. Initial multiple sequence alignment was done with the GCG Pileup program, and phylogenetic analysis was performed by using the Phylogenetic Analysis Using Parsimony (and other methods) program (version 4.0) (20).

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