Volume 6, Number 6—December 2000
Research
Hantavirus Pulmonary Syndrome Associated with Monongahela Virus, Pennsylvania
Luther V. Rhodes*
, Cinnia Huang†, Angela J. Sanchez§, Stuart T. Nichol§, Sherif R. Zaki§, Thomas G. Ksiazek§, J.G. Humphreys¶, James J. Freeman*, and Kenneth R. Knecht*
, Cinnia Huang†, Angela J. Sanchez§, Stuart T. Nichol§, Sherif R. Zaki§, Thomas G. Ksiazek§, J.G. Humphreys¶, James J. Freeman*, and Kenneth R. Knecht*Table 1
Laboratory Data
| Complete blood count | Patient 1 | Patient 2 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Date | 3/10/97 | 3/12/97 | 3/13/97 | 11/13/97 | 11/14/97 | 11/15/97 | 11/16/97 | ||
| Time (hrs) | 2057 | 1443 | 0700 | 1100 | 1356 | 1856 | 0450 | 0400 | 0925 |
| Leukocytes | 4,300 | 4,800 | 12,100 | 10,900 | 4,400 | 5,100 | 8,000 | 11,100 | 15,700 |
| Polymorphonuclear cells (%) | 58 | 60 | 45 | 53 | 67 | 62 | 81 | 58 | 73 |
| Bands (%) | 3 | 23 | 34 | 27 | 0 | 9 | N/Da | 11 | N/D |
| Immunoblasts | 4 | 1 | 3 | 5 | 2 | N/D | N/D | N/D | N/D |
| Platelets | 100,000 | 27,000 | 13,000 | 2,000 | 128,000 | 74,000 | 59,000 | ||
| Hematocrit | 45.9 | 47.0 | 55.5 | 44.0 | 47.4 | 46.4 | 48.2 | ||
| Creatinine (mg/dL) | 0.8 | 0.8 | 1.9 | 1.2 | 1.1 | 1.0 | |||
aN/D, not done.
1Three fragments of the virus genome were amplified by nested polymerase chain reaction to allow generation of nucleotide (nt) sequences 393 nt in length for an S segment region encoding the N protein, 259 nt in length for an M segment region (M-G1) encoding the G1 protein, and 205 nt in length for an M segment region (M-G2) encoding the G2 protein (12). Sequence data were analyzed by using the Wisconsin GCG Package (version 9.1) software. Initial multiple sequence alignment was done with the GCG Pileup program, and phylogenetic analysis was performed by using the Phylogenetic Analysis Using Parsimony (and other methods) program (version 4.0) (20).
