Emerging Infectious Disease ISSN: 1080-6059

Molecular Evidence of Clonal Vibrio parahaemolyticus Pandemic Strains

Nandini Roy Chowdhury*, Soumen Chakraborty*, Thandavarayan Ramamurthy*, Mitsuaki Nishibuchi†, Shinji Yamasaki*‡, Yoshifumi Takeda§, and Gopinath Balakrish Nair*

Author affiliations: *National Institute of Cholera and Enteric Diseases, Calcutta, India; †Center for Southeast Asian Studies, Kyoto University, Kyoto, Japan; ‡Research Institute, International Medical Center of Japan, Shinjuku-ku, Tokyo, Japan; §National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan

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Figure 1

Southern blot hybridization of BglI-digested Vibrio parahaemolyticus chromosomal DNA with rRNA probe. 1a-1c, ribotype patterns of the O3:K6, O4:K68, and O1:K untypeable (KUT) strains, respectively, isolated from different countries. 1d, ribotype pattern of the nonpandemic strains isolated from different countries and belonging to various serotypes (Table 2). The last three lanes indicate the pattern of the representative O3:K6(KX-V225), O4:K68(KX-V563), and O1:KUT(KX-V737) strains, respectively.

Figure 1. Southern blot hybridization of BglI-digested Vibrio parahaemolyticus chromosomal DNA with rRNA probe. 1a-1c, ribotype patterns of the O3:K6, O4:K68, and O1:K untypeable (KUT) strains, respectively, isolated from different countries. 1d, ribotype pattern of the nonpandemic strains isolated from different countries and belonging to various serotypes (Table 2). The last three lanes indicate the pattern of the representative O3:K6(KX-V225), O4:K68(KX-V563), and O1:KUT(KX-V737) strains, respectively. Positions of λ-HindIII molecular-size markers run on the same gel are indicated on the left margin. *=Strains isolated from travelers to the Kansai Airport from the respective places.

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