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Volume 7, Number 1—February 2001

Dispatch

Tracking Cryptosporidium parvum by Sequence Analysis of Small Double-Stranded RNA

Lihua XiaoComments to Author , Josef Limor, Caryn Bern, and Altaf A. Lal
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA

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Table

Cryptosporidium parvum isolates used in this study.

Isolate Host Source Genotype ds-RNA sequence typea
6 Calf Ohio, 1996b Bovine B
7 Calf Ohio, 1996 Bovine M
11 Calf Ohio, 1996 Bovine G
16 Calf Ohio, 1996 Bovine B
28 Calf Ohio, 1996 Bovine G
45 Calf Ohio, 1996 Bovine D
46 Calf Ohio, 1996 Bovine G
49 Calf Ohio, 1996 Bovine G
50 Calf Ohio, 1996 Bovine D
51 Calf Ohio, 1996 Bovine G
53 Calf Ohio, 1996 Bovine B
57 Calf Ohio, 1996 Bovine D
3 Calf Oklahoma, 1996 Bovine D
29 Calf Oklahoma, 1996 Bovine D
89 Calf Pennsylvania, 1997 Bovine D
21 Calf Idaho, 1996 Bovine F
37 Calf Utah, 1996 Bovine M
1346 Calf California, 1999 Bovine G
1347 Calf California, 1999 Bovine G
43 Human via calf Maryland, 1996c Bovine A
Beltsville Calf Maryland, 1996 Bovine H
AUCP Calf Alabama, 1996 Bovine A
KSU-1 Calf Kansas, 1996 Bovine A
1676 Human Peru, 1995d Human J
1677 Human Peru, 1996 Human J
1683 Human Peru, 1997 Human I
1684 Human Peru, 1997 Human I
1685 Human Peru, 1997 Human I
1902 Human Kenya, 1999e Human Q
1904 Human Kenya, 1999 Human Q
1905 Human Kenya, 1999 Human R
1911 Human Kenya, 1999 Human R
1927 Human Kenya, 1999 Human R
1935 Human Kenya, 1999 Human R
HGA5 Human Georgia, 1995 Human N
HNO3 Human New Orleans, 1997f Human N
HNO6 Human New Orleans, 1997 Human E
HNO23 Human New Orleans, 1998 Human P
HNO27 Human New Orleans, 1998 Human N
HNO30 Human New Orleans, 1998 Human N
HNO32 Human New Orleans, 1998 Human N
HNO35 Human New Orleans, 1998 Human O
HNO52 Human New Orleans, 1999 Human N
HMOB1 Human Minnesota outbreak, 1997 Bovine C
HMOB3 Human Minnesota outbreak, 1997 Bovine C
HMOB4 Human Minnesota outbreak, 1997 Bovine C
HMOB5 Human Minnesota outbreak, 1997 Bovine C
HWA1 Human Spokane outbreak, 1997 Human L
HWA3 Human Spokane outbreak, 1997 Human L
HWA4 Human Spokane outbreak, 1997 Human L
HWA5 Human Spokane outbreak, 1997 Human L
HWA6 Human Spokane outbreak, 1997 Human L
HDC1 Human Washington, DC, outbreak, 1998 Human K
HDC2 Human Washington, DC, outbreak, 1998 Human K
HDC6 Human Washington, DC, outbreak, 1998 Human K
HDC7 Human Washington, DC, outbreak, 1998 Human K
HDC14 Human Washington, DC, outbreak, 1998 Human K
HDC16 Human Washington, DC, outbreak, 1998 Human K
HDC23 Human Washington, DC, outbreak, 1998 Human K
HDC25 Human Washington, DC, outbreak, 1998 Human K

ads = double-stranded
bThe Ohio bovine samples were collected from four dairy farms in central Ohio over a 12-month period.
cThe dates for laboratory isolates (Beltsville, KSU-1, and AUCP) were the dates that oocyst passages were harvested for DNA extraction.
dPeruvian samples 1683, 1684, and 1685 were taken from the same patient on different days.
eKenyan samples were collected from patients visiting two hospitals in Nairobi.
fThe New Orleans samples were from HIV-positive patients.

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