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Volume 7, Number 3—June 2001

Research

Molecular Epidemiology of Serogroup A Meningitis in Moscow, 1969 to 1997

Mark Achtman*Comments to Author , Arie van der Ende†, Peixuan Zhu*, Irina S. Koroleva‡, Barica Kusecek*, Giovanna Morelli*, Ilse G.A. Schuurman†, Norbert Brieske*, Kerstin Zurth*, Natalya N. Kostyukova§, and Alexander E. Platonov‡
Author affiliations: *Max-Planck Institut für Molekulare Genetik, Berlin, Germany; †Academic Medical Center, Amsterdam, the Netherlands; ‡Central Research Institute of Epidemiology, Moscow, Russia; §N.F. Gamaleya Institute of Epidemiology and Microbiology, Moscow, Russia

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Figure 2

UPGMA tree of band differences between random amplified polymorphic DNA (RAPD) patterns from 103 serogroup A strains from Moscow. RAPD tests were performed with primers 1254, 1281, NM03, and NM04 as described (7), generating four patterns for each strain. The combination of the differences between these patterns was used to generate a UPGMA tree with the program GelCompar (Applied Maths, Kortrijk, Belgium), as described (7). Subgroup designations are indicated within the tree. The strain designa

Figure 2. . UPGMA tree of band differences between random amplified polymorphic DNA (RAPD) patterns from 103 serogroup A strains from Moscow. RAPD tests were performed with primers 1254, 1281, NM03, and NM04 as described (7), generating four patterns for each strain. The combination of the differences between these patterns was used to generate a UPGMA tree with the program GelCompar (Applied Maths, Kortrijk, Belgium), as described (7). Subgroup designations are indicated within the tree. The strain designations and the sequence type (ST) assignments from MLST analysis are shown at the right. For subgroup III bacteria, dark gray rectangles indicate pgm3 and light gray rectangles indicate pgm19. Strain Z4714 is indicated as belonging to subgroup IV because RAPD does not distinguish between subgroups IV-1 and IV-2.

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