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Volume 7, Number 3—June 2001

Research

Outbreak of Human Monkeypox, Democratic Republic of Congo, 1996 to 1997

Yvan J.F. Hutin*, R. Joel Williams*, Philippe Malfait†, Richard Pebody†, Vladamir N. Loparev*, Susan L. Ropp*, Mariangelli Rodriguez*, Janice C Knight*, Florimont K. Tshioko‡, Ali S Khan*, Mark V. Szczeniowski‡, and Joseph J. Esposito*Comments to Author 
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †European Programme for Intervention Epidemiology Training, Brussels, Belgium; ‡World Health Organization, Geneva, Switzerland

Main Article

Figure 2

Phylogenetic inference relationships of the open reading frames encoding the viral hemagglutinin protein of various monkeypox virus isolates and selected strains of vaccinia, variola, and cowpox viruses. Nucleotide sequences of polymerase chain reaction-generated amplicons were analyzed using PAUP parsimony analysis software version 3.1.1, as described (10). Parsimony analysis used 5,000 bootstraps and weighted the sequences for a transition-transversion ratio of 2 (bootstrap confidence interval

Figure 2. . Phylogenetic inference relationships of the open reading frames encoding the viral hemagglutinin protein of various monkeypox virus isolates and selected strains of vaccinia, variola, and cowpox viruses. Nucleotide sequences of polymerase chain reaction-generated amplicons were analyzed using PAUP parsimony analysis software version 3.1.1, as described (10). Parsimony analysis used 5,000 bootstraps and weighted the sequences for a transition-transversion ratio of 2 (bootstrap confidence intervals shown on branches).

Main Article

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