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Volume 7, Number 4—August 2001
THEME ISSUE
West Nile Virus
West Nile Virus

Rapid Determination of HLA B*07 Ligands from the West Nile Virus NY99 Genome

Anne S. De Groot*†Comments to Author , Caitlin Saint-Aubin*, Andrew Bosma*, Hakima Sbai*, James Rayner†, and William Martin†
Author affiliations: *Brown University, Providence, Rhode Island, USA; †EpiVax, Inc., Providence, Rhode Island, USA

Main Article

Table 3

West Nile virus T2B7 binding assay results

Fluorescence ratio (peptide/negative control)
Fluorescence ratio comparisons (p value)
Peptide no. AA sequence EBPa Avg. fold inc. @200b Avg. MFI 10 20 200 control -10 control -20 control -200 Summary
WNB7 0004 AVKDELNTLL 47.77 1.0 842.5 0.91 0.89 0.90 0.021 0.007 0.009 None
WNB7 0005 APAYSFNCLG 47.03 1.2 743.5 0.96 1.09 1.14 0.511 0.127 0.020 Weak
WNB7 0006 AAKKKGASLL 45.3 1.2 708.1 1.01 1.04 1.19 0.717 0.100 0.000 Weak
WNB7 0007 NPMILAAGLI 36.60 1.1 944.6 0.89 0.92 1.00 0.008 0.051 0.949 None
WNB7 0008 IPAGFEPEML 36.02 1.9 1,207.4 1.03 1.18 1.89 0.468 0.000 0.000 Moderate
WNB7 0009 TPAAPSYTLK 35.68 1.5 954.5 1.03 1.13 1.55 0.372 0.001 0.000 Moderate
WNB7 0010 VPCRGQDELV 32.57 1.0 658.6 1.05 1.06 1.04 0.053 0.015 0.118 None
WNB7 0011 GPGHEEPQLV 32.35 1.0 848.9 1.01 1.03 0.98 0.759 0.521 0.689 None
WNB7 0012 AGMLLLSLLL 31.17 -- -- -- -- -- -- -- -- --
WNB7 0013 EPPEGVKYVL 31.07 1.1 681.7 1.08 1.10 1.06 0.004 0.000 0.023 Weak
WNB7 0014 MPAILIALLV 30.23 -- -- -- -- -- -- -- -- --
WNB7 0015 KPTGSASSLV 28.79 1.7 1,070.2 1.04 1.08 1.68 0.243 0.012 0.000 Weak
WNB7 0017 RPRWIDARVY 23.99 1.9 1,714.0 1.18 1.43 1.97 0.003 0.000 0.000 Strong
WNB7 0018 VPGTKIAGML 23.10 1.7 1,088.4 0.98 1.06 1.54 0.533 0.058 0.000 Weak
WNB7 0019 RPQRHDEKTL 22.49 2.8 1,644.6 1.32 1.57 2.77 0.000 0.000 0.000 Strong
WNB7 0020 SPHRVPNYNL 22.40 2.5 1,607.9 1.37 1.59 2.44 0.000 0.000 0.000 Strong
WNB7 0021 IPMTIAGLMF 22.32 -- -- -- -- -- -- -- -- --
WNB7 0023 RPAADGRTVM 20.89 1.5 979.1 1.68 2.01 2.90 0.000 0.000 0.000 Strong
WNB7 0024 TPGLRCLNLD 20.73 1.0 849.6 1.09 1.11 1.01 0.037 0.015 0.821 Weak
WNB7 0025 SVNMTSQVLL 20.09 -- -- -- -- -- -- -- -- --
WNB7 3399 PEDIDCWCTK 0.00 1.1 990.3 0.97 0.96 0.94 0.448 0.396 0.145 None
WNB7 3403 PETPQGLAKI 0.00 1.0 588.9 1.02 1.02 0.99 0.484 0.395 0.716 None
WNB7 3411 PFPESNSPIS 0.00 1.0 626.7 0.90 0.86 0.90 0.020 0.001 0.019 None
WNB7 3415 PRTNTILEDN 0.00 0.9 778.6 0.92 0.91 0.90 0.041 0.020 0.008 None
HIV-1 B7 1291 GPGHKARVLA 28.0 2.2 1,423.8 1.16 1.20 1.72 0.002 0.000 0.000 Strong
1.5 1,370.9 1.12 1.14 1.78 0.000 0.000 0.000 Strong
1.6 1,003.9 0.98 1.06 1.66 0.720 0.195 0.000 Weak

aEBP = estimated binding potential; MFI = mean fluorescence index and T2B7 binding assay results for each of the peptides.
bAverage fold increase in fluorescence of cells incubated with peptide at 200 µg/mL.

Main Article

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