Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis
Figure 3. Schematic structure of the prn1 gene, showing the position of the primers used in the allele-specific amplification assay (1.), and the primers and probes used in the fluorescence resonance energy transfer probe assay (2.). Proportions of the gene are not drawn to scale. F = fluorescein; LC = LC red 640; and P = phosphate.