Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis
Figure 5. A. Melting curves from the allele-specific amplification assay, showing the presence of amplified products from prn1-5 and the absence of amplification from prn6-8 and the negative control. B. Corresponding melting peaks derived from the melting curve. Prn1 represents the prn1-5 types; prn7 represents prn6-8 types and the negative control.