Waterborne Outbreak of Tularemia Associated with Crayfish Fishing
Pedro Anda* , Javier Segura del Pozo*†, José María Díaz García‡, Raquel Escudero*, F. Javier García Peña§, M. Carmen López Velasco‡, Ricela E. Sellek*, M. Rosario Jiménez Chillarón‡, Luisa P. Sánchez Serrano*, and J. Fernando Martínez Navarro*
Author affiliations: *Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; †Public Health Department, Alcalá de Henares, Madrid, Spain; ‡Cuenca Public Health Department, Cuenca, Spain; §Ministry of Agriculture and Fisheries, Algete, Madrid, Spain
Figure 5. . A) Sequence alignment of a 75-nt fragment of the 16S rRNA gene of different bacterial species (nucleotide position 1113 to 1188 as for Escherichia coli). The signature nucleotide (nt 1153), which allows differentiation between F. tularensis palaearctica and the other members of the Francisella genus, is set in bold face. B) Sequence comparison of the regions corresponding to F11- and F5-specific Francisella tularensis primers between F. tularensis and other bacterial species. Asterisks depict Francisella spp.-specific nucleotides. Sequences generated in this study were deposited in GenBank with accession numbers of AF227312 for the amplicon from water, AF227313 for the fragment amplified from crayfish stomach, and AF227314 for the human lymph node aspirate. Sequences generated in this study were compared with those of Salmonella Typhimurium (X80681), Escherichia coli (AE000406), Legionella pneumophila (M36023), F. tularensis biovar tularensis (Z21932), F. tularensis biovar palaearctica (L26086), F. novicida (L26084), and F. philomiragia (L26085).
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