Volume 8, Number 1—January 2002
Research
Prevalence and Genetic Profiling of Virulence Determinants of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Beef, and Humans, Calcutta, India
Asis Khan*, Shinji Yamasaki*†, Toshio Sato†, Thandavarayan Ramamurthy*, Amit Pal*, Simanti Datta*, Nandini Roy Chowdhury*, Suresh Chandra Das‡, Asim Sikdar‡, Teizo Tsukamoto§, Sujit Kumar Bhattacharya*, Yoshifumi Takeda¶, and Gopinath Balakrish Nair*#
Table 6
Serotype, phenotypic and genotypic traits of Shiga toxin-producing Escherichia coli strains, India
| Strain no. | Serotype | Origin | Genotype (PCR/colony hybridization) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chromosomal genes |
Plasmid genes |
Phenotype | ||||||||||||
| stx1 | stx2 | eae | hlyA | etpD | katP | espP | Hemolysis | SFa | Verob | Stx1 | Stx2 | |||
| AK-40 | O11:H8 | Human | +/+ | -/- | +/+ | +/+ | -/- | +/+ | -/+ | E-hly | + | ND | ND | ND |
| AK-11 | ONT:H18 | Human | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | E-hly | + | + | + | - |
| AK-18 | O156:H7 | Human | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | - | + | + | + | - |
| AK-27 | ONT:H19 | Human | +/+ | -/- | -/- | -/- | -/- | +/+ | -/- | - | + | + | + | - |
| AK-30 | O7:H6 | Human | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | - | + | + | + | - |
| AK-32 | ONT:NM | Human | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | - | ND | ND | ND | ND |
| AK-33 | O159:H12 | Human | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | E-hly | + | + | + | - |
| AK-26 | O159:H9 | Human | -/- | +/+ | -/- | -/- | -/- | -/- | -/- | - | + | + | - | + |
| AK-29 | O110:H16 | Human | -/- | +/+ | -/- | -/- | -/- | -/- | -/- | - | + | + | - | + |
| AK-14 | ONT:H14 | Human | +/+ | +/+ | -/- | +/+ | -/- | -/- | -/+ | E-hly | + | + | + | + |
| AK-28 | O43:H2 | Human | +/+ | +/+ | -/- | +/+ | -/- | -/- | -/- | - | + | + | + | + |
| AK-31 | O96:H19 | Human | +/+ | +/+ | -/- | -/- | -/- | -/- | -/- | - | ND | ND | ND | ND |
| P-33-2-26 | ONT:H2 | Cow | +/+ | -/- | +/+ | +/+ | +/+ | +/+ | -/- | E-hly | + | + | + | - |
| AK-38 | O103:H2 | Cow | +/+ | -/- | +/+ | +/+ | +/+ | +/+ | -/- | - | + | - | - | - |
| P-179-101 | O146:H1 | Cow | +/+ | -/- | -/- | +/+ | -/- | -/- | -/+ | E-hly | + | + | + | - |
| C-45-3-19 | O149:HNT | Cow | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | - | + | + | + | - |
| AK-35 | O22:H16 | Cow | +/+ | -/- | -/- | -/- | -/- | +/+ | -/- | - | + | + | + | - |
| AK-39 | O9B:H5 | Cow | +/+ | -/- | -/- | -/- | -/- | -/- | +/+ | E-hly | + | + | + | - |
| C-5-1-13 | O82:H8 | Cow | -/- | +/+ | -/- | +/+ | -/- | -/- | -/+ | E-hly | + | + | - | + |
| AK-1 | O2:H25 | Cow | -/- | +/+ | -/- | -/+ | -/- | +/+ | -/- | - | + | + | - | - |
| AK-37 | O22:NM | Cow | -/- | +/+ | -/- | +/+ | -/- | -/- | +/+ | - | ND | ND | ND | ND |
| C-2-1-15 | O28ac:H21 | Cow | +/+ | +/+ | -/- | +/+ | -/- | -/- | -/+ | E-hly | + | + | + | + |
| C-3-2-25 | ONT:H34 | Cow | +/+ | +/+ | -/- | +/+ | -/- | -/- | -/+ | E-hly | + | + | + | + |
| C-3-3-42-6 | ONT:H19 | Cow | +/+ | +/+ | -/- | -/- | -/- | -/- | -/+ | E-hly | + | + | + | + |
| P-33-2-4 | O88:HNT | Cow | +/+ | +/+ | -/- | +/+ | -/- | -/- | -/+ | E-hly | + | + | + | + |
| AK-36 | O159:H12 | Cow | +/+ | +/+ | -/- | -/- | -/- | -/- | -/- | E-hly | + | + | + | + |
| AK-16 | ONT:H21 | Beef | +/+ | -/- | +/- | -/- | -/- | -/- | -/- | - | + | + | + | - |
| AK-12 | O20:H12 | Beef | +/+ | +/+ | -/- | -/- | -/- | -/- | -/- | ND | + | + | + | - |
| AK-13 | O110:HNT | Beef | +/+ | +/+ | -/- | -/- | -/- | -/- | -/- | ND | + | + | + | - |
| AK-17 | O172:NM | Beef | +/+ | +/+ | -/- | +/+ | -/- | +/+ | -/+ | E-hly | + | + | + | + |
aFermentation of sorbitol.
bVero cell cytotoxicity.
NT, Not typable; ND, Not determined; NM, nonmotile.
