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Volume 8, Number 10—October 2002
THEME ISSUE
Bioterrorism-related Anthrax

Bioterrorism-related Anthrax

Molecular Subtyping of Bacillus anthracis and the 2001 Bioterrorism-Associated Anthrax Outbreak, United States

Alex R. Hoffmaster*Comments to Author , Collette C. Fitzgerald*, Efrain Ribot*, Leonard W. Mayer*, and Tanja Popovic*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

Main Article

Table 2

MLVA and pagA genotyping of Bacillus anthracis isolatesa,b

B. anthracis strain No. strains vrrA vrrB1 vrrB2 vrrC1 vrrC2 CG3 pXO1 pXO2 MLVA type PA genotype
Outbreak-associated 317 313 229 153 583 532 158 123 141 62 I
Ames 1 313 229 153 583 532 158 123 141 62 I
NH (2000032764) 1 301 229 153 538 604 158 132 139 78 VI
NH (2000032760) 1 313 229 153 538 604 158 123 139 Newe VI
RI (2000032763) 1 313 229 162 538 604 158 132 139 71 VI
RI (2000032761) 1 313 229 162 538 604 158 132 139 71 VI
MA (2000032762) 1 313 229 153 538 604 158 132 143 Newe VI
State A
(2002017388) 1 313 229 162 613 604 153 137 Pasteurc NAd
Pasteur (LRN) 1 313 229 162 613 604 153 137 Pasteurc NAd
Country B (2002007581) 1 313 229 162 613 532 158 129 141 45 I
Country B (2002007648) 1 313 229 162 613 532 158 129 141 45 I
Country B (2002007649) 1 313 229 162 613 604 153 137 Pasteurc NAd
Country B (2002007650) 1 313 229 162 583 532 158 129 _ Sternec I
Country B (2002007651) 1 313 229 162 583 532 158 129 _ Sternec I

aMLVA, multiple-locus variable-number tandem repeat analysis; pagA, protective antigen gene; PA, protective antigen; –, loci not detected; NA, not applicable.
bAllele size for each VNTR locus is shown in addition to the MLVA and PA genotypes.
cNo MLVA genotype assigned due to the lack of one of the virulence plasmids (pXO1 or pXO2).
dpagA not present in pXO1-cured strains and thus could not be assigned a PA genotype.
eNew combination of alleles resulting in a new genotype. Genotype no. to be assigned at a later date.

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