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Volume 8, Number 12—December 2002

Research

Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway

Joo-Sung Yang*1, Mathura P. Ramanathan*1, Karuppiah Muthumani*, Andrew Y. Choo*, Sung-Ha Jin*, Qian-Chun Yu*, Daniel S. Hwang*, Daniel K. Choo*, Mark D. Lee*, Kesen Dang*, J. Joseph Kim†, David B. Weiner*Comments to Author , and WaixingTang
Author affiliations: *University of Pennsylvania, Philadelphia, Pennsylvania, USA; †Viral Genomix, Inc., Philadelphia, Pennsylvania, USA;

Main Article

Figure 4

Mitochondria transmembrane potential and caspase activities measurement. HeLa-CD4 cells were transfected with pcWNV-Cp-DJY (a) or pcDNA3.1 (b), and their mitochondria transmembrane potential was measured after 48 h by ultraviolete illumination. A colorimetric caspase activity assay was performed with pcWNV-Cp-DJY– or pcDNA3.1-transfected cells for caspase-3 (c) or caspase-9 (d) activity. As a specificity control, the inhibitor LEHD-FMK for caspase-9 was added to the reactions along with relevant substrate (d). A specific positive control was used for assay validation (magnification: 1000X [a and b]).

Figure 4. Mitochondria transmembrane potential and caspase activities measurement. HeLa-CD4 cells were transfected with pcWNV-Cp-DJY (a) or pcDNA3.1 (b), and their mitochondria transmembrane potential was measured after 48 h by ultraviolete illumination. A colorimetric caspase activity assay was performed with pcWNV-Cp-DJY– or pcDNA3.1-transfected cells for caspase-3 (c) or caspase-9 (d) activity. As a specificity control, the inhibitor LEHD-FMK for caspase-9 was added to the reactions along with relevant substrate (d). A specific positive control was used for assay validation (magnification: 1000X [a and b]).

Main Article

1 These authors contributed equally.

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