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Volume 8, Number 2—February 2002
Perspective

Traditional and Molecular Techniques for the Study of Emerging Bacterial Diseases: One Laboratory’s Perspective

Pierre Houpikian and Didier RaoultComments to Author 
Author affiliations: Unité des Rickettsies, Faculté de Médecine de Marseille, Marseille, France

Main Article

Table 2

Key issues for isolating main emerging bacteria

Medium
Conditions for incubation
Group Axenic specific medium Living system (embryonated egg,
cell line) Low temperature
(<37°C) O2 and CO2 conditions Extended incubation
Alpha1 Proteo-
bacteriae Ehrlichia sp.
Rickettsia sp.
Chlamydia sp. ELB agent
(“Rickettsia felis”) (28°C) Ehrlichia sp.
Rickettsia sp.
Alpha2 Proteo-
bacteriae Afipia sp. Afipia sp.
Bartonella sp. Bartonella bacilliformis (28°C) Bartonella sp.
Spirochetae Borrelia sp. Treponema pallidum
Delta-Xi Proteo-
bacteriae Campylobacter sp. (microaerophilic)
Helicobacter sp. (microaerophilic) Helicobacter pylori
Gamma Proteo-
bacteriae Legionella sp. Legionella sp. Yersinia pestis
Mycobacteria Mycobacterium sp. Mycobacterium leprae Mycobacterium malmoense (microaerophilic) Mycobacterium sp.
Mycoplasmas Mycoplasma sp. Mycoplasma fermentans
Gram-positive bacteria Tropheryma whipplei Clostridium difficile
(anaerobic) Tropheryma whipplei

Main Article

Page created: April 18, 2012
Page updated: April 18, 2012
Page reviewed: April 18, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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