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Volume 8, Number 2—February 2002

Research

Broad-Range Bacterial Detection and the Analysis of Unexplained Death and Critical Illness

Simo Nikkari*†, Fred A. Lopez*†, Paul W. Lepp*, Paul R. Cieslak‡, Stephen Ladd-Wilson‡, Douglas Passaro§, Richard Danila¶, and David A. Relman*Comments to Author 
Author affiliations: *Stanford University School of Medicine, Stanford, California, USA; †VA Palo Alto Health Care System, Palo Alto, California, USA; ‡Oregon Health Division, Department of Human Services, Portland, Oregon, USA; §California Emerging Infections Program, Berkeley, California, USA; and ¶Minnesota Department of Health, Minneapolis, Minnesota, USA;

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Figure

Phylogenetic analysis of the bacterial 16S rDNA sequences obtained from cases XEB44 and XMN22. The tree was rooted with Staphylococcus aureus and Escherichia coli as outgroups and constructed with a maximum-likelihood algorithm using 468 homologous sequence positions that were selected from a sequence dataset of 497 total positions. Streptococcus pneumoniae clinical isolates sequenced for this study are marked as SF10175, SF10314, and SF10014. GenBank database accession numbers for published sequences are given in parentheses. All six sequences from the case XCA73 cerebrospinal fluid were identical to those of the S. pneumoniae reference strain (accession no. AJ001246). PCR = polymerase chain reaction.

Figure. Phylogenetic analysis of the bacterial 16S rDNA sequences obtained from cases XEB44 and XMN22. The tree was rooted with Staphylococcus aureus and Escherichia coli as outgroups and constructed with a maximum-likelihood algorithm using 468 homologous sequence positions that were selected from a sequence dataset of 497 total positions. Streptococcus pneumoniae clinical isolates sequenced for this study are marked as SF10175, SF10314, and SF10014. GenBank database accession numbers for published sequences are given in parentheses. All six sequences from the case XCA73 cerebrospinal fluid were identical to those of the S. pneumoniae reference strain (accession no. AJ001246). PCR = polymerase chain reaction.

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