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Volume 8, Number 4—April 2002

Research

Genetic and Antigenic Analysis of the First A/New Caledonia/20/99-like H1N1 Influenza Isolates Reported in the Americas

Luke T. Daum*, Linda C. Canas*, Catherine B. Smith†, Alexander Klimov†, William Huff*, William Barnes*, and Kenton L. Lohman*Comments to Author 
Author affiliations: *Brooks Air Force Base, San Antonio, Texas, USA; †Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

Main Article

Table 1

Characterization of Influenza A H1N1 (HA1) Sequencing Primers

Primer/nt position Sequence % GCa Tmb(°C)
H1F-272 5′-AATCATGGTCCTACATTG-3′ 38.9 57
H1F-734 5′-ACTACTACTGGACTCTGC-3′ 50 58
H1R-365 5′-TTCCTCATACTCGGCGAA-3′ 50 58
H1R-1110 5′-CCATCCATCTATCATTCC-3′ 44.4 55

aGC= (Guanosine (G) + Cytidine (C))/ (Adenosine (A)+ Thymidine (T)+ Guanosine (G) + Cytidine (C)) x 100
bTm= 81.5 + 16.6 * log [Na+] + 41 * (#G + #C)/length – 500/length where [Na+]= 0.1M.

Main Article

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