Volume 8, Number 4—April 2002
O157:H7 Shiga Toxin-Producing Escherichia coli Strains Associated with Sporadic Cases of Diarrhea in São Paulo, Brazil
To the Editor: Shiga toxin-producing Escherichia coli (STEC) strains are associated with a spectrum of diseases ranging from mild to severe bloody diarrhea and complications such as hemolytic uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (1). Since STEC was linked with hemorrhagic colitis in 1982 (2), strains—particularly serotype O157:H7—have been associated with numerous outbreaks and many sporadic cases of infections worldwide. STEC is now a major cause of foodborne disease, primarily in the United States, Canada, Japan, and Europe (1,3). Although most sporadic cases and outbreaks have been reported from developed countries, human infections associated with STEC strains have also been described in Latin American countries, including Argentina and Chile (3). In Brazil, STEC infections have been related to sporadic cases of nonbloody diarrhea caused by non-O157 strains (4,5); serotype O157:H7 has not been previously isolated from human infections in our country.
We report the characterization of three O157:H7 strains isolated in São Paulo State, Brazil. The first strain was identified among a laboratory collection of 2,573 E. coli strains that were retrospectively analysed and isolated from patients with diarrhea in São Paulo State, from 1976 through 1999, at the Central Laboratory of Instituto Adolfo Lutz (IAL). This strain was isolated in 1990 from an 18-year-old patient with diarrheal disease who had AIDS. The two other O157 strains were recently isolated from a 4-year-old girl with bloody diarrhea and from an adult with severe diarrhea. Both patients were admitted to the same hospital at Campinas, São Paulo State, in June and July 2001, respectively. The strains, isolated by routine diagnostic procedures on MacConkey agar plates, were presumptively identified as E. coli O157 by standard methods with specific O157 antiserum. These last two strains were confirmed as sorbitol-negative E. coli O157 at the IAL Regional Laboratory at Campinas and were sent to the IAL Central Laboratory for further characterization.
The three O157 E. coli strains underwent biochemical identification and serotyping by standard methods. Enterohemolysin production was determined according to Beutin et al. (6). Polymerase chain reaction assays were used to detect the stx1 and stx2 genes (7), and colony hybridization assays with specific DNA probes for stx1 , stx2 and eae genes were performed as described (8). Cytotoxicity to Vero cells was assayed as described (4). The strains were characterized as sorbitol-negative O157:H7, containing the stx2 and eae sequences. The enterohemolytic phenotype and production of Stx were also observed in all isolates.
To our knowledge, these O157:H7 STEC strains are the first to be associated with human diseases in Brazil. Cultivation of stool specimens in sorbitol MacConkey agar is strongly recommended for screening O157 strains, and, indeed, all three strains were isolated from MacConkey agar plates. Laboratories should attempt to examine stool specimens from all patients (children and adults) with HUS, severe diarrhea (nonbloody and bloody stools) requiring hospitalization, or both, as well as from patients reporting a history of bloody diarrhea.
Despite the importance of O157:H7 serotype in causing life-threatening complications such as HUS and the isolation of this serotype from clinical specimens in São Paulo State, the relatively low prevalence of this serotype in healthy dairy and beef cattle in Brazil (9), as well as the occurrence of other non-O157 STEC strains associated with human infections (4,5,10), suggest that E. coli O157:H7 may be not as frequent as non-O157 STEC strains in our country.
- Griffin P, Tauxe RV. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev. 1991;13:60–98.
- Riley LW, Remis RS, Helgerson SD, McGee HB, Wells JG, Davis BD, Hemorrhagic colitis associated with a rare Escherichia coli O157:H7 serotype. N Engl J Med. 1983;308:681–5.
- Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998;11:142–201.
- Giraldi R, Guth BEC, Trabulsi LR. Production of Shiga-like toxin among Escherichia coli strains and other bacteria isolated from diarrhea in São Paulo, Brazil. J Clin Microbiol. 1990;28:1460–2.
- Guth BEC, Ramos SRTS, Cerqueira AMF, Andrade JRC, Gomes TAT. Characterization of Shiga toxin-producing Escherichia coli (STEC) strains isolated from children in São Paulo, Brazil. 4th International Symposium and Workshop on Shiga toxin (Verocytotoxin)-producing Escherichia coli infections—VTEC 2000. Kyoto, Japan, 2000. p. 149.
- Beutin L, Prada J, Zimmermann S, Stephan R, Orskov I, Orskov F. Enterohemolysin, a new type of hemolysin produced by some strains of enteropathogenic E. coli (EPEC). Zentralbl Bakteriol Mikrobiol Hyg [A]. 1988;267:576–88.
- Pollard DR, Johnson W. M, Lior H, Tyler SD, Rozee KR. Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction. J Clin Microbiol. 1990;28:540–5.
- Gonçalves AG, Campos LC, Gomes TAT, Rodrigues J, Sperandio V, Whittam T, Virulence properties and clonal structure of strains of Escherichia coli O119 serotypes. Infect Immun. 1997;▪▪▪:652034–40.
- Cerqueira AMF, Guth BEC, Joaquim RM, Andrade JRC. High occurrence of Shiga toxin-producing Escherichia coli (STEC) in healthy cattle in Rio de Janeiro State, Brazil. Vet Microbiol. 1999;70:111–21.
- Irino K, Gomes TAT, Vaz TMI, Kano E, Kato MAMF, Dias AMG, Prevalence of Shiga toxin and intimin gene sequences among Escherichia coli of serogroups O26, O55, O111, O119 and O157 isolated in São Paulo, Brazil. In: Abstracts of the 4th International Symposium and Workshop on Shiga toxin (Verocytotoxin)-producing Escherichia coli infections. Kyoto, Japan, 2000. p.107.
Suggested citation: Irino K, Vaz TMI, Kato MAMF, Naves ZVF, Lara RR, Carvalho Marco ME, et al. O157:H7 Shiga Toxin-Producing Escherichia coli Strains Associated with Sporadic Cases of Diarrhea in São Paulo, Brazil. Emerg Infect Dis. [serial on the Internet]. 2002 Apr [date cited]. Available from http://wwwnc.cdc.gov/eid/article/8/4/01-0490
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
- Page created: July 15, 2010
- Page last updated: July 15, 2010
- Page last reviewed: July 15, 2010
- Centers for Disease Control and Prevention,
National Center for Emerging and Zoonotic Infectious Diseases (NCEZID)
Office of the Director (OD)