Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 9, Number 1—January 2003

Dispatch

Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic Escherichia coli

Catalina López-Saucedo*, Jorge F. Cerna*, Nicolas Villegas-Sepulveda*, Rocío Thompson*, F. Raul Velazquez†, Javier Torres†, Phillip I. Tarr‡§, and Teresa Estrada-García*Comments to Author 
Author affiliations: *Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México D.F., México; †Hospital de Pediatría, México D.F., México; ‡Children’s Hospital and Regional Medical Center, Seattle, Washington, USA; §University of Washington School of Medicine, Seattle, Washington, USA

Main Article

Figure

Polymerase chain reaction (PCR) products of each locus. Lane 1: sizes of the seven PCR products of each locus in base pairs, obtained when using a DNA mix of the four reference strains and the primers mix. PCR products obtained by using DNA of enterotoxigenic Escherichia coli, Shiga-toxin–producing E. coli, enteropathogenic E. coli, and enteroinvasive E. coli (lanes 2–5, respectively). Lane 6–11: PCR products obtained when using DNA of patients’ isolates and the primers mix. Lanes 12–15: PCR pro

Figure. Polymerase chain reaction (PCR) products of each locus. Lane 1: sizes of the seven PCR products of each locus in base pairs, obtained when using a DNA mix of the four reference strains and the primers mix. PCR products obtained by using DNA of enterotoxigenic Escherichia coli, Shiga-toxin–producing E. coli, enteropathogenic E. coli, and enteroinvasive E. coli (lanes 2–5, respectively). Lane 6–11: PCR products obtained when using DNA of patients’ isolates and the primers mix. Lanes 12–15: PCR products obtained when using DNA of food isolates and the primers mix. Lane 16: 1 kb molecular weight marker in base pairs.

Main Article

TOP