Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic Escherichia coli
Figure. Polymerase chain reaction (PCR) products of each locus. Lane 1: sizes of the seven PCR products of each locus in base pairs, obtained when using a DNA mix of the four reference strains and the primers mix. PCR products obtained by using DNA of enterotoxigenic Escherichia coli, Shiga-toxin–producing E. coli, enteropathogenic E. coli, and enteroinvasive E. coli (lanes 2–5, respectively). Lane 6–11: PCR products obtained when using DNA of patients’ isolates and the primers mix. Lanes 12–15: PCR products obtained when using DNA of food isolates and the primers mix. Lane 16: 1 kb molecular weight marker in base pairs.