Electron Microscopy for Rapid Diagnosis of Emerging Infectious Agents1
Figure 3. Three methods for efficient collection of vesicular and blister fluids for diagnostic electron microscopic. A. The contents of a vesicle are collected into the barrel of a needle. B. After the blister is opened, a coated electron microscopic grid is touched to the fluid and air-dried (direct electron microscopic). C. A glass microscope slide is touched directly to an unroofed lesion and a smear prepared. Samples are then placed in rigid containers for transport to the electron microscopic laboratory.